Alkaline Phosphatase, Intestinal Antibody (23GB3645)

Novus Biologicals | Catalog # NBP3-51119

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Knockdown Validated

Label

Unconjugated

Antibody Source

Monoclonal Rabbit IgG Clone # 23GB3645
View all Alkaline Phosphatase, Intestinal Primary Antibodies »

Product Specifications

Immunogen

A synthesized peptide derived from human Alkaline Phosphatase, Intestinal (Uniprot: P09923)

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Alkaline Phosphatase, Intestinal Antibody (23GB3645)

Alkaline Phosphatase, Intestinal Antibody (23GB3645)

Immunocytochemistry/ Immunofluorescence: Alkaline Phosphatase, Intestinal Antibody (23GB3645)[NBP3-51119] -

Immunocytochemical staining of HepG2 cells with Alkaline Phosphatase, Intestinal antibody (1:1,000). Nuclei were stained blue with DAPI; Alkaline Phosphatase, Intestinal was stained magenta with Alexa Fluor(R) 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 um.
Alkaline Phosphatase, Intestinal Antibody (23GB3645)

Flow Cytometry: Alkaline Phosphatase, Intestinal Antibody (23GB3645)[NBP3-51119] -

Validation of Alkaline Phosphatase, Intestinal knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with Alkaline Phosphatase, Intestinal antibody (1:2,000) and analyzed using BD flow cytometer.
Alkaline Phosphatase, Intestinal Antibody (23GB3645)

Flow Cytometry: Alkaline Phosphatase, Intestinal Antibody (23GB3645)[NBP3-51119] -

Flow cytometric analysis of Alkaline Phosphatase, Intestinalexpression in HepG2 cells using Alkaline Phosphatase, Intestinal antibody (1:2,000). Green, isotype control; red, Alkaline phosphatase.

Applications for Alkaline Phosphatase, Intestinal Antibody (23GB3645)

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Knockdown Validated

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.4), 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Alkaline Phosphatase, Intestinal

Alkaline phosphatase removes phosphate groups from the 5' end of DNA and RNA, and from proteins, at high pH. Most mammals have 4 different isozymes - placental, placental-like, intestinal and non-tissue specific (found in liver, kidney and bone).

Long Name

Alkaline Phosphatase, Intestinal Type

Alternate Names

alkaline phosphatase, intestinal, Alkaline phosphomonoesterase, ALPI, EC 3.1.3.1, Glycerophosphatase, IAP, Intestinal alkaline phosphatase, intestinal-type alkaline phosphatase, Kasahara isozyme

Gene Symbol

ALPI

Additional Alkaline Phosphatase, Intestinal Products

Product Documents for Alkaline Phosphatase, Intestinal Antibody (23GB3645)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Alkaline Phosphatase, Intestinal Antibody (23GB3645)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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