ALKBH5 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-82188

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human

Cited:

Human, Mouse

Predicted:

Rat (96%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Simple Western, Knockdown Validated

Cited:

Immunohistochemistry-Paraffin, Western Blot, IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all ALKBH5 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: EKLKSMTSRDNYKAGSREAAAAAAAAVAAAAAAAAAAEPYPVSGAKRKYQEDSDPERSDYEEQQLQKEEEARKVKSGIRQMRLFSQDECAKIEARIDEVVSRAEKGLYNEHTVD

Reactivity Notes

Use in Mouse reported in scientific literature (PMID:32329191).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

51 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ALKBH5 Antibody - BSA Free

Western Blot: ALKBH5 Antibody [NBP1-82188]

Western Blot: ALKBH5 Antibody [NBP1-82188]

Western Blot: ALKBH5 Antibody [NBP1-82188] - Analysis in human cell line U-87 MG.
Immunohistochemistry-Paraffin: ALKBH5 Antibody [NBP1-82188]

Immunohistochemistry-Paraffin: ALKBH5 Antibody [NBP1-82188]

ALKBH5-Antibody-Immunohistochemistry-Paraffin-NBP1-82188-img0023.jpg
Western Blot: ALKBH5 Antibody [NBP1-82188]

Western Blot: ALKBH5 Antibody [NBP1-82188]

Western Blot: ALKBH5 Antibody [NBP1-82188] - Analysis of ALKBH5 in MDA-MB-231 human breast cancer subclones using anti-ALKBH5 antibody. Image from verified customer review.
Immunohistochemistry-Paraffin: ALKBH5 Antibody [NBP1-82188]

Immunohistochemistry-Paraffin: ALKBH5 Antibody [NBP1-82188]

Immunohistochemistry-Paraffin: ALKBH5 Antibody [NBP1-82188] - Staining of human liver shows weak to moderate nuclear positivity in hepatocytes.
Immunohistochemistry-Paraffin: ALKBH5 Antibody [NBP1-82188]

Immunohistochemistry-Paraffin: ALKBH5 Antibody [NBP1-82188]

Immunohistochemistry-Paraffin: ALKBH5 Antibody [NBP1-82188] - Staining of human fallopian tube shows moderate to strong nuclear positivity in glandular cells.
Immunohistochemistry-Paraffin: ALKBH5 Antibody [NBP1-82188]

Immunohistochemistry-Paraffin: ALKBH5 Antibody [NBP1-82188]

Immunohistochemistry-Paraffin: ALKBH5 Antibody [NBP1-82188] - Staining of human kidney shows nuclear positivity.
Immunohistochemistry-Paraffin: ALKBH5 Antibody [NBP1-82188]

Immunohistochemistry-Paraffin: ALKBH5 Antibody [NBP1-82188]

Immunohistochemistry-Paraffin: ALKBH5 Antibody [NBP1-82188] - Staining of human testis shows moderate to strong nuclear positivity in cells in seminiferous ducts.
Knockdown Validated: ALKBH5 Antibody [NBP1-82188]

Western Blot: ALKBH5 Antibody [NBP1-82188]

ALKBH5-Antibody-Western-Blot-NBP1-82188-img0022.jpg
ALKBH5 Antibody

Western Blot: ALKBH5 Antibody [NBP1-82188] -

Western Blot: ALKBH5 Antibody [NBP1-82188] - ZNF217 & ALKBH5 regulate NANOG & KLF4 expression via modulation of m6A levels. J. MCF-7 subclones exposed to 20% or 1% O2 for 48 h & immunoblot assays performed. Image collected & cropped by CiteAb from the following publication (https://www.oncotarget.com/lookup/doi/10.18632/oncotarget.11743), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for ALKBH5 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000

Simple Western

1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Reviewed Applications

Read 1 review rated 5 using NBP1-82188 in the following applications:

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ALKBH5

ALKBH5, RNA demethylase ALKBH5, ALKB homolog 5 (44 kDa) is a dioxygenase enzyme which demethylates RNA in a reaction requiring oxygen, alpha-ketoglutarate, and iron. ALKBH5 and fat mass and obesity-associated protein (FTO) are involved in the demethylation of N6-methyladenosine or m6A mRNA, the most common post-translational modification in eukaryotic mRNA. m6A mRNA modifications are very dynamic; they are introduced into the mRNA by m6A methyltransferases (e.g., METTL3/14 and KIAA1429), removed by erasers such as FTO and ALKBH5, and decoded by m6A binding proteins (e.g., IGF2BP1 and YTHDF1/2/3). m6A mRNA modifications play roles in regulating mRNA splicing, processing, translation and stability. m6A mRNA modifications are also associated with pathological states including cancer (1, 2).

ALKBH5 has been implicated in various physiological and pathophysiological processes. In mice, ALKBH5 is highly expressed in the testes where loss of ALKBH5 expression leads to decreased spermatogenesis, abnormal spermatozoa and increased apoptosis (3). In glioblastoma, ALKBH5 overexpression promotes stemness and tumor progression (2). In breast cancer cells under hypoxic conditions, HIF-1 alpha and HIF-2 alpha induce the expression of ALKH5 and leads to increased demethylation and expression of Nanog thus promoting breast cancer stem cell properties (4).

References

1. Chen, X. Y., Zhang, J., & Zhu, J. S. (2019). The role of m6A RNA methylation in human cancer. Molecular Cancer. https://doi.org/10.1186/s12943-019-1033-z

2. Deng, X., Su, R., Weng, H., Huang, H., Li, Z., & Chen, J. (2018). RNA N 6 -methyladenosine modification in cancers: Current status and perspectives. Cell Research. https://doi.org/10.1038/s41422-018-0034-6

3. Meyer, K. D., & Jaffrey, S. R. (2014). The dynamic epitranscriptome: N6-methyladenosine and gene expression control. Nature Reviews Molecular Cell Biology. https://doi.org/10.1038/nrm3785

4. Zhang, C., Samanta, D., Lu, H., Bullen, J. W., Zhang, H., Chen, I.,... Semenza, G. L. (2016). Hypoxia induces the breast cancer stem cell phenotype by HIF-dependent and ALKBH5-mediated m6A-demethylation of NANOG mRNA. Proceedings of the National Academy of Sciences of the United States of America. https://doi.org/10.1073/pnas.1602883113

Long Name

RNA demethylase ALKBH5

Alternate Names

ABH5, EC 1.14.11.53, OFOXD1

Entrez Gene IDs

54890 (Human)

Gene Symbol

ALKBH5

UniProt

Q6P6C2 (Human)

Additional ALKBH5 Products

Product Documents for ALKBH5 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for ALKBH5 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for ALKBH5 Antibody - BSA Free

Customer Reviews for ALKBH5 Antibody - BSA Free (1)

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  • Name: Anonymous
    Application: Western Blot
    Sample Tested:
    Species: Human
    Verified Customer | Posted 05/29/2015
    Analysis of AKBH5 expression in MDA-MB-231 human breast cancer subclones
    ALKBH5 Antibody - BSA Free NBP1-82188

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