alpha-2C Adrenergic R/ADRA2C Antibody (S330A-80) - BSA Free

Novus Biologicals | Catalog # NBP2-22397

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # S330A-80

Format

BSA Free
View all alpha-2C Adrenergic R/ADRA2C Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptide amino acids 442- 462 (QDFRRSFKHILFRRRRRGFRQ, cytoplasmic C-terminus) of human Alpha-2C adrenergic receptor

Localization

Cell Membrane

Specificity

Detects 50kDa or larger (possibly due to dimerization). Does not cross-react with other adrenergic receptors.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for alpha-2C Adrenergic R/ADRA2C Antibody (S330A-80) - BSA Free

Western Blot: alpha-2C Adrenergic R/ADRA2C Antibody (S330A-80) [NBP2-22397]

Western Blot: alpha-2C Adrenergic R/ADRA2C Antibody (S330A-80) [NBP2-22397]

Western Blot: alpha-2C Adrenergic R/ADRA2C Antibody (S330A-80) [NBP2-22397] - Western Blot analysis of Monkey COS cells transfected with HA-tagged Alpha-2C showing detection of ~50 kDa Alpha-2C Adrenergic Receptor protein using Mouse Anti-Alpha-2C Adrenergic Receptor Monoclonal Antibody, Clone S330A-80 (NBP2-22397). Lane 1: Molecular Weight Ladder. Lane 2: Monkey COS cells transfected with HA-tagged Alpha-2C. Load: 15 ug. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-Alpha-2C Adrenergic Receptor Monoclonal Antibody (NBP2-22397) at 1:200 for 16 hours at 4C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 1 hour RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~50 kDa.
Immunocytochemistry/ Immunofluorescence: alpha-2C Adrenergic R/ADRA2C Antibody (S330A-80) [NBP2-22397]

Immunocytochemistry/ Immunofluorescence: alpha-2C Adrenergic R/ADRA2C Antibody (S330A-80) [NBP2-22397]

Immunocytochemistry/Immunofluorescence: alpha-2C Adrenergic R/ADRA2C Antibody (S330A-80) [NBP2-22397] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Alpha-2C Adrenergic Receptor Monoclonal Antibody, Clone S330A-80 (NBP2-22397). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-Alpha-2C Adrenergic Receptor Monoclonal Antibody (NBP2-22397) at 1:100 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) Alpha-2C Adrenergic Receptor Antibody (D) Composite.
Immunocytochemistry/ Immunofluorescence: alpha-2C Adrenergic R/ADRA2C Antibody (S330A-80) [NBP2-22397]

Immunocytochemistry/ Immunofluorescence: alpha-2C Adrenergic R/ADRA2C Antibody (S330A-80) [NBP2-22397]

Immunocytochemistry/Immunofluorescence: alpha-2C Adrenergic R/ADRA2C Antibody (S330A-80) [NBP2-22397] - Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Alpha-2C Adrenergic Receptor Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cell Membrane, Nucleus. Magnification: 60X.
Immunocytochemistry/ Immunofluorescence: alpha-2C Adrenergic R/ADRA2C Antibody (S330A-80) [NBP2-22397]

Immunocytochemistry/ Immunofluorescence: alpha-2C Adrenergic R/ADRA2C Antibody (S330A-80) [NBP2-22397]

Immunocytochemistry/Immunofluorescence: alpha-2C Adrenergic R/ADRA2C Antibody (S330A-80) [NBP2-22397] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Alpha-2C Adrenergic Receptor Monoclonal Antibody, Clone S330A-80 (NBP2-22397). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Alpha-2C Adrenergic Receptor Monoclonal Antibody (NBP2-22397) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cell Membrane, Nucleus. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) Alpha-2C Adrenergic Receptor Antibody. (D) Composite.

Applications for alpha-2C Adrenergic R/ADRA2C Antibody (S330A-80) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:10-1:2000

Western Blot

1:1000
Application Notes
1 ug/ml of Alpha-2C Adrenergic Receptor Antibody was sufficient for detection of Alpha2C Adrenergic Receptor in 20 ug of COS cells transiently transfected with HA-tagged Alpha- lysate and assayed by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary Antibody.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS (pH 7.4), 50% Glycerol

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: alpha-2C Adrenergic R/ADRA2C

The alpha-2-adrenergic receptor is a member of the G protein-coupled receptor superfamily. There are 3 highly homologous subtypes: alpha2A, alpha2B, and alpha2C. These receptors have a critical role in regulating neurotransmitter release from sympathetic nerves and from adrenergic neurons in the central nervous system. Alpha2C is required for normal presynaptic control of transmitter release from sympathetic nerves in the heart and from central noradrenergic neurons.

Long Name

Alpha-2C Adrenergic Receptor

Alternate Names

a-2C AdrenergicR, ADRA2C, ADRA2L2, ADRA2RL2, ADRARL2, alpha2C AdrenergicR

Gene Symbol

ADRA2C

UniProt

P18825 (Human)

Additional alpha-2C Adrenergic R/ADRA2C Products

Product Documents for alpha-2C Adrenergic R/ADRA2C Antibody (S330A-80) - BSA Free

Certificate of Analysis

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Product Specific Notices for alpha-2C Adrenergic R/ADRA2C Antibody (S330A-80) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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