Aminopeptidase N/CD13 Antibody (23GB3360)

Novus Biologicals | Catalog # NBP3-51087

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Knockdown Validated

Label

Unconjugated

Antibody Source

Monoclonal Rabbit IgG Clone # 23GB3360
View all Aminopeptidase N/CD13 Primary Antibodies »

Product Specifications

Immunogen

A synthesized peptide derived from human Aminopeptidase N/CD13 (Uniprot: P15144)

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Aminopeptidase N/CD13 Antibody (23GB3360)

Aminopeptidase N/CD13 Antibody (23GB3360)

Immunocytochemistry/ Immunofluorescence: Aminopeptidase N/CD13 Antibody (23GB3360)[NBP3-51087] -

Immunocytochemical staining of HT-1080 cells with Aminopeptidase N/CD13 antibody (1:1,000). Nuclei were stained blue with DAPI; Aminopeptidase N/CD13 was stained magenta with Alexa Fluor(R) 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 um.
Aminopeptidase N/CD13 Antibody (23GB3360)

Flow Cytometry: Aminopeptidase N/CD13 Antibody (23GB3360)[NBP3-51087] -

Flow cytometric analysis of Aminopeptidase N/CD13expression in HT-1080 cells using Aminopeptidase N/CD13 antibody (1:2,000). Green, isotype control; red, CD13.
Aminopeptidase N/CD13 Antibody (23GB3360)

Western Blot: Aminopeptidase N/CD13 Antibody (23GB3360)[NBP3-51087] -

Western blotting analysis using Aminopeptidase N/CD13 antibody (1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using FeQ(TM) ECL Substrate Kit.
Aminopeptidase N/CD13 Antibody (23GB3360)

Western Blot: Aminopeptidase N/CD13 Antibody (23GB3360)[NBP3-51087] -

Western blotting analysis using Aminopeptidase N/CD13 antibody (1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using FeQ(TM) ECL Substrate Kit.

Applications for Aminopeptidase N/CD13 Antibody (23GB3360)

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Knockdown Validated

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.4), 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Aminopeptidase N/CD13

Alanine aminopeptidase (CD13) is expressed on the majority of peripheral blood monocytes and granulocytes. CD13 is also expressed by the majority of acute myeloid leukemias, chronic myeloid leukemias in myeloid blast crisis, a smaller percentage of lymphoid leukemias and myeloid cell lines. CD13 is also found in several types of non hematopoietic cells such as fibroblasts and endothelial cells and in a soluble form in blood plasma. CD13 is not expressed on B cells, T cells, platelets or erythrocytes. In the mouse, CD13 is a non-covalently linked homodimer of approximately 150kD subunits expressed by a variety of cells including monocytes, macrophages, dendritic cell and veiled cells. CD13 plays a role in biologically active peptide metabolism, in the control of growth and differentiation, in phagocytosis and in bactericidal/tumoricidal activities. CD13 also serves as a receptor for human coronaviruses.

CD13 is commonly used as a biomarker to detect damage to the kidneys, and that may be used to help diagnose certain kidney disorders. Therefore, CD13 antibodies are useful tools for cancer and renal disorder research.

Alternate Names

Aminopeptidase M, ANPEP, APN, CD13, gp150, PEPN

Gene Symbol

ANPEP

Additional Aminopeptidase N/CD13 Products

Product Documents for Aminopeptidase N/CD13 Antibody (23GB3360)

Certificate of Analysis

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Product Specific Notices for Aminopeptidase N/CD13 Antibody (23GB3360)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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