Angiopoietin-1 Antibody (MM0019-26B10) - Azide and BSA Free
Novus Biologicals | Catalog # NB110-85464
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Scientific Data Images for Angiopoietin-1 Antibody (MM0019-26B10) - Azide and BSA Free
Immunohistochemistry-Paraffin: Angiopoietin-1 Antibody (MM0019-26B10) - Azide and BSA Free [NB110-85464]
Immunohistochemistry-Paraffin: Angiopoietin-1 Antibody (MM0019-26B10) [NB110-85464] - Angiopoietin 1 Antibody (MM0019-26B10) [NB110-85464] - Formalin fixed and paraffin embedded human placental tissue section is subjected to IHC with anti human Angiopoietin-1.Applications for Angiopoietin-1 Antibody (MM0019-26B10) - Azide and BSA Free
Immunohistochemistry
Immunohistochemistry-Paraffin
Western Blot
Reviewed Applications
Read 2 reviews rated 3 using NB110-85464 in the following applications:
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Background: Angiopoietin-1
Additional Angiopoietin-1 Products
Product Documents for Angiopoietin-1 Antibody (MM0019-26B10) - Azide and BSA Free
Certificate of Analysis
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Product Specific Notices for Angiopoietin-1 Antibody (MM0019-26B10) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Citations for Angiopoietin-1 Antibody (MM0019-26B10) - Azide and BSA Free
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Customer Reviews for Angiopoietin-1 Antibody (MM0019-26B10) - Azide and BSA Free (2)
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Customer Images
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Application: ImmunofluorescenceSample Tested:Species: OtherVerified Customer | Posted 08/26/2014indirect immunofluorescence data with anti-Ang1 on chicken comb
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Application: Immunohistochemistry-FrozenSample Tested: Frozen human skin sectionsSpecies: HumanVerified Customer | Posted 07/12/2013
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
FAQs for Angiopoietin-1 Antibody (MM0019-26B10) - Azide and BSA Free
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Q: How do you dissolve the lyophilized Ab? Water or PBS? The datasheet for NB110-85464 just says "Buffer: Lyophilized" - - in Water? Buffer? Concentration of Buffer? NB110-85467 says Lyophilized from a solution of PBS - again how to dissolve the Ab - which PBS and what concentration?
A: The IgG fraction of culture supernatant was purified by Protein G affinity chromatography and lyophilized from a 0.2 um filtered solution in phosphate-buffered saline (PBS). 100 ug antibody is lyophilized from 40 ul of 1 x PBS. We suggest reconstituting the antibody with 500 ul sterile 1 x PBS (or to be perfect, with 40 ul H2O plus 460 ul PBS), to give a final concentration of 200 ug/ml. Alternatively, you may reconstitute the antibody in 40 ul H2O if a small volume is preferred.