Annexin V Antibody (23GB650)
Novus Biologicals | Catalog # NBP3-52019
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Key Product Details
Species Reactivity
Applications
Label
Antibody Source
Product Specifications
Immunogen
Clonality
Host
Isotype
Scientific Data Images for Annexin V Antibody (23GB650)
Immunocytochemistry/ Immunofluorescence: Annexin V Antibody (23GB650)[NBP3-52019] -
Immunocytochemical staining of HT-1080 cells with Annexin V antibody (1:1,000). Nuclei were stained blue with DAPI; Annexin V was stained magenta with Alexa Fluor(R) 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 um.
Flow Cytometry: Annexin V Antibody (23GB650)[NBP3-52019] -
Validation of Annexin V knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with Annexin V antibody (1:2,000) and analyzed using CytoFLEX.
Flow Cytometry: Annexin V Antibody (23GB650)[NBP3-52019] -
Flow cytometric analysis of Annexin Vexpression in HT-1080 cells using Annexin V antibody (isotype control; red, Annexin V.
Western Blot: Annexin V Antibody (23GB650)[NBP3-52019] -
Western blotting analysis using Annexin V antibody. Annexin Vexpression in wild type (WT) and Annexin VshRNA knockdown (KD) HeLa cells with 30 ug of total cell lysates. beta-Tubulin serves as a loading control. The blot was incubated with Annexin V antibody(1:10,000) and HRP-conjugated goat anti rabbit secondary antibody (1:50,000) respectively. Image was developed using FeQ(TM) ECL Substrate Kit.Applications for Annexin V Antibody (23GB650)
Flow Cytometry
Immunocytochemistry/ Immunofluorescence
Knockdown Validated
Western Blot
Flow Cytometry Panel Builder
Bio-Techne Knows Flow Cytometry
Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.
Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Formulation
Preservative
Concentration
Shipping
Stability & Storage
Background: Annexin V
Alternate Names
Gene Symbol
Additional Annexin V Products
Product Documents for Annexin V Antibody (23GB650)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Annexin V Antibody (23GB650)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
Customer Reviews for Annexin V Antibody (23GB650)
There are currently no reviews for this product. Be the first to review Annexin V Antibody (23GB650) and earn rewards!
Have you used Annexin V Antibody (23GB650)?
Submit a review and receive an Amazon gift card!
$25/€18/£15/$25CAN/¥2500 for a review with an image
$10/€7/£6/$10CAN/¥1110 for a review without an image
Submit a review
Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- 7-Amino Actinomycin D (7-AAD) Cell Viability Flow Cytometry Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Extracellular Membrane Flow Cytometry Protocol
- Flow Cytometry Protocol for Cell Surface Markers
- Flow Cytometry Protocol for Staining Membrane Associated Proteins
- Flow Cytometry Staining Protocols
- Flow Cytometry Troubleshooting Guide
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Intracellular Flow Cytometry Protocol Using Alcohol (Methanol)
- Intracellular Flow Cytometry Protocol Using Detergents
- Intracellular Nuclear Staining Flow Cytometry Protocol Using Detergents
- Intracellular Staining Flow Cytometry Protocol Using Alcohol Permeabilization
- Intracellular Staining Flow Cytometry Protocol Using Detergents to Permeabilize Cells
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Propidium Iodide Cell Viability Flow Cytometry Protocol
- Protocol for Liperfluo
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Characterization of Human Th22 Cells
- Protocol for the Characterization of Human Th9 Cells
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- Protocol: Annexin V and PI Staining by Flow Cytometry
- Protocol: Annexin V and PI Staining for Apoptosis by Flow Cytometry
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Fluorokine Flow Cytometry Kits
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
FAQs for Annexin V Antibody (23GB650)
-
Q: I am studying apoptosis in adherent cells. Can I trypsinize the cells when using Annexin V-FITC to stain the cells?
A: Adherent cells undergoing apoptosis automatically detach from the plate and may not require trypsinization. If there are attached cells, they may be trypsinized without affecting phosphatidylserine (PS).
-
Q: I do not have access to flow cytometer in my building. Can I stain for Annexin V and propidium iodide (PI), fix the cells, then analyze them in a different facility?
A: Fixing the cells can affect cell permeability, enhancing the uptake of Annexin V and PI intracellularly. As a result, the profile of fixed cells may be different from unfixed cells, and the use of appropriate controls is recommended.
-
Q: I am studying apoptosis in adherent cells. Can I trypsinize the cells when using Annexin V-FITC to stain the cells?
A: Adherent cells undergoing apoptosis automatically detach from the plate and may not require trypsinization. If there are attached cells, they may be trypsinized without affecting phosphatidylserine (PS).
-
Q: I do not have access to flow cytometer in my building. Can I stain for Annexin V and propidium iodide (PI), fix the cells, then analyze them in a different facility?
A: Fixing the cells can affect cell permeability, enhancing the uptake of Annexin V and PI intracellularly. As a result, the profile of fixed cells may be different from unfixed cells, and the use of appropriate controls is recommended.