Annexin V Antibody - BSA Free
Novus Biologicals | Catalog # NBP2-38248
![Immunocytochemistry/ Immunofluorescence: Annexin V Antibody [NBP2-38248]](https://resources.rndsystems.com/images/products/Annexin-V-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-38248-img0004.jpg "Immunocytochemistry/ Immunofluorescence: Annexin V Antibody [NBP2-38248]")
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
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Scientific Data Images for Annexin V Antibody - BSA Free
Immunocytochemistry/ Immunofluorescence: Annexin V Antibody [NBP2-38248]
Immunocytochemistry/Immunofluorescence: Annexin V Antibody [NBP2-38248] - Staining of human cell line PC-3 shows localization to nuclear membrane. Antibody staining is shown in green.
Western Blot: Annexin V Antibody [NBP2-38248] -
Western Blot: Annexin V Antibody [NBP2-38248] - Analysis in human cell line SK-MEL-30.![Annexin V Antibody - BSA Free Immunohistochemistry-Paraffin: Annexin V Antibody [NBP2-38248]](https://resources.rndsystems.com/images/products/nbp2-38248_-immunohistochemistry-paraffin-639173121457732894.jpg "Immunohistochemistry-Paraffin: Annexin V Antibody [NBP2-38248]")
![Annexin V Antibody - BSA Free Immunohistochemistry-Paraffin: Annexin V Antibody [NBP2-38248]](https://resources.rndsystems.com/images/products/nbp2-38248_-immunohistochemistry-paraffin-639173123135809680.jpg "Immunohistochemistry-Paraffin: Annexin V Antibody [NBP2-38248]")
Immunohistochemistry-Paraffin: Annexin V Antibody [NBP2-38248]
Staining of human skeletal muscle shows low positivity in myocytes as expected.![Annexin V Antibody - BSA Free Immunohistochemistry-Paraffin: Annexin V Antibody [NBP2-38248]](https://resources.rndsystems.com/images/products/nbp2-38248_-immunohistochemistry-paraffin-639173125120104773.jpg "Immunohistochemistry-Paraffin: Annexin V Antibody [NBP2-38248]")
Immunohistochemistry-Paraffin: Annexin V Antibody [NBP2-38248]
Staining of human placenta shows moderate membranous positivity in trophoblastic cells.![Annexin V Antibody - BSA Free Immunohistochemistry-Paraffin: Annexin V Antibody [NBP2-38248]](https://resources.rndsystems.com/images/products/nbp2-38248_-immunohistochemistry-paraffin-639173125190985668.jpg "Immunohistochemistry-Paraffin: Annexin V Antibody [NBP2-38248]")
Immunohistochemistry-Paraffin: Annexin V Antibody [NBP2-38248]
Staining of human prostate shows moderate membranous positivity in glandular cells.![Annexin V Antibody - BSA Free Immunohistochemistry-Paraffin: Annexin V Antibody [NBP2-38248]](https://resources.rndsystems.com/images/products/nbp2-38248_-immunohistochemistry-paraffin-639173132732761134.jpg "Immunohistochemistry-Paraffin: Annexin V Antibody [NBP2-38248]")
Immunohistochemistry-Paraffin: Annexin V Antibody [NBP2-38248]
Staining of human fallopian tube shows moderate positivity in cilia in glandular cells.Applications for Annexin V Antibody - BSA Free
Immunocytochemistry/ Immunofluorescence
Immunohistochemistry-Paraffin
Western Blot
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Background: Annexin V
Additional Annexin V Products
Product Documents for Annexin V Antibody - BSA Free
Certificate of Analysis
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Product Specific Notices for Annexin V Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
FAQs for Annexin V Antibody - BSA Free
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Q: I am studying apoptosis in adherent cells. Can I trypsinize the cells when using Annexin V-FITC to stain the cells?
A: Adherent cells undergoing apoptosis automatically detach from the plate and may not require trypsinization. If there are attached cells, they may be trypsinized without affecting phosphatidylserine (PS).
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Q: I do not have access to flow cytometer in my building. Can I stain for Annexin V and propidium iodide (PI), fix the cells, then analyze them in a different facility?
A: Fixing the cells can affect cell permeability, enhancing the uptake of Annexin V and PI intracellularly. As a result, the profile of fixed cells may be different from unfixed cells, and the use of appropriate controls is recommended.
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Q: I am studying apoptosis in adherent cells. Can I trypsinize the cells when using Annexin V-FITC to stain the cells?
A: Adherent cells undergoing apoptosis automatically detach from the plate and may not require trypsinization. If there are attached cells, they may be trypsinized without affecting phosphatidylserine (PS).
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Q: I do not have access to flow cytometer in my building. Can I stain for Annexin V and propidium iodide (PI), fix the cells, then analyze them in a different facility?
A: Fixing the cells can affect cell permeability, enhancing the uptake of Annexin V and PI intracellularly. As a result, the profile of fixed cells may be different from unfixed cells, and the use of appropriate controls is recommended.