AP2S1 Antibody (JE47-37)

Novus Biologicals | Catalog # NBP3-32041

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # JE47-37 expressed in HEK293
View all AP2S1 Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptide within Human AP2S1 aa 1-50 / 142. (Uniprot: P53680)

Localization

Cell membrane.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

17 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for AP2S1 Antibody (JE47-37)

AP2S1 Antibody (JE47-37) Western Blot: AP2S1 Antibody (JE47-37) [NBP3-32041]

Western Blot: AP2S1 Antibody (JE47-37) [NBP3-32041]

Western blot analysis on different lysates with NBP3-32041 at 1/1,000 dilution. Lane 1: K-562 cell lysate (30 µg/Lane) Lane 2: MCF7 cell lysate (30 µg/Lane) Lane 3: NIH/3T3 cell lysate (30 µg/Lane) Lane 4: PC-12 cell lysate (30 µg/Lane) Lane 5: Mouse spleen tissue lysate (40 µg/Lane) Lane 6: Rat kidney tissue lysate (40 µg/Lane) Predicted band size: 17 kDa Observed band size: 14 kDa Exposure time: 1 minute 6 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP3-32041 at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG [HRP] Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.

Applications for AP2S1 Antibody (JE47-37)

Application
Recommended Usage

Western Blot

1:1000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

1*TBS (pH7.4), 0.05% BSA and 40% Glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: AP2S1

One of two major clathrin-associated adaptor complexes, AP-2, is a heterotetramer which is associated with the plasma membrane. This complex is composed of two large chains, a medium chain, and a small chain. This gene encodes the small chain of this complex. Alternative splicing has been observed in this gene and results in two known transcripts.

Alternate Names

Adapter-related protein complex 2 sigma subunit, Adaptor protein complex AP-2 subunit sigma, adaptor-related protein complex 2, sigma 1 subunit, AP17HA2 17 kDa subunit, AP-2 complex subunit sigma, CLAPS2AP17-DELTA, Clathrin assembly protein 2 small chain, Clathrin coat assembly protein AP17, Clathrin coat-associated protein AP17, clathrin-associated/assembly/adaptor protein, small 2 (17kD), Plasma membrane adaptor AP-2 17 kDa protein, sigma2-adaptin

Gene Symbol

AP2S1

Additional AP2S1 Products

Product Documents for AP2S1 Antibody (JE47-37)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for AP2S1 Antibody (JE47-37)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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