Apolipoprotein E/ApoE Antibody (GT27711) - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-13692

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Sandwich ELISA

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # GT27711

Format

Azide and BSA Free
View all Apolipoprotein E/ApoE Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human Apolipoprotein E/ApoE. The exact sequence is proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Description

Centrifuge briefly prior to opening.

Scientific Data Images for Apolipoprotein E/ApoE Antibody (GT27711) - Azide and BSA Free

ELISA: Apolipoprotein E/ApoE Antibody (GT27711) [NBP3-13692]

ELISA: Apolipoprotein E/ApoE Antibody (GT27711) [NBP3-13692]

ELISA: Apolipoprotein E/ApoE Antibody (GT27711) [NBP3-13692] - Indirect ELISA analysis performed by coating plate with recombinant full-length human apolipoprotein E (400-6.25 ng/mL). Coated protein was probed with Apolipoprotein E/ApoE antibody [GT27711] (NBP3-13692) (1 ug/mL). Mouse IgG antibody (HRP) (NBP2-19382) (1:10000) was used to detect bound primary antibody.
Sandwich ELISA: Apolipoprotein E/ApoE Antibody (GT27711) [NBP3-13692] - Sandwich ELISA detection of recombinant full-length human apolipoprotein E using Apolipoprotein E/ApoE antibody [GT27711] (NBP3-13692) as capture antibody at concentration of 5 ug/mL and Apolipoprotein E/ApoE antibody [C2C3], C-term as detection antibody at concentration of 1 ug/mL. Rabbit IgG antibody (HRP) (NBP2-19301) was diluted at 1:10000 and used to detect the primary antibody.
Apolipoprotein E/ApoE Antibody (GT27711)

Western Blot: Apolipoprotein E/ApoE Antibody (GT27711) [NBP3-13692] -

Western Blot: Apolipoprotein E/ApoE Antibody (GT27711) [NBP3-13692] - Human plasma (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Apolipoprotein E/ApoE antibody [GT27711] (NBP3-13692) diluted at 1:25000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
Apolipoprotein E/ApoE Antibody (GT27711)

Western Blot: Apolipoprotein E/ApoE Antibody (GT27711) [NBP3-13692] -

Western Blot: Apolipoprotein E/ApoE Antibody (GT27711) [NBP3-13692] - Rat plasma (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Apolipoprotein E/ApoE antibody [GT27711] (NBP3-13692) diluted at 1:25000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
Apolipoprotein E/ApoE Antibody (GT27711) - Azide and BSA Free

Immunohistochemistry-Paraffin: Apolipoprotein E/ApoE Antibody (GT27711) [NBP3-13692] -

Apolipoprotein E antibody [GT27711] detects Apolipoprotein E protein by immunohistochemical analysis.
Sample: Paraffin-embedded rat tissues.
Apolipoprotein E stained by Apolipoprotein E antibody [GT27711] (NBP3-13692) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Apolipoprotein E/ApoE Antibody (GT27711) - Azide and BSA Free

Immunohistochemistry-Paraffin: Apolipoprotein E/ApoE Antibody (GT27711) [NBP3-13692] -

Apolipoprotein E antibody [GT27711] detects Apolipoprotein E protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse tissues.
Apolipoprotein E stained by Apolipoprotein E antibody [GT27711] (NBP3-13692) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Apolipoprotein E/ApoE Antibody (GT27711) - Azide and BSA Free

Immunohistochemistry-Paraffin: Apolipoprotein E/ApoE Antibody (GT27711) [NBP3-13692] -

Apolipoprotein E antibody [GT27711] detects Apolipoprotein E protein by immunohistochemical analysis.
Sample: Paraffin-embedded rat tissues.
Apolipoprotein E stained by Apolipoprotein E antibody [GT27711] (NBP3-13692) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Apolipoprotein E/ApoE Antibody (GT27711) - Azide and BSA Free

Immunohistochemistry-Paraffin: Apolipoprotein E/ApoE Antibody (GT27711) [NBP3-13692] -

Apolipoprotein E antibody [GT27711] detects Apolipoprotein E protein by immunohistochemical analysis.
Sample: Paraffin-embedded human tissues.
Apolipoprotein E stained by Apolipoprotein E antibody [GT27711] (NBP3-13692) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Apolipoprotein E/ApoE Antibody (GT27711) - Azide and BSA Free

Immunohistochemistry-Paraffin: Apolipoprotein E/ApoE Antibody (GT27711) [NBP3-13692] -

Apolipoprotein E antibody [GT27711] detects Apolipoprotein E protein by immunohistochemical analysis.
Sample: Paraffin-embedded human tissues.
Apolipoprotein E stained by Apolipoprotein E antibody [GT27711] (NBP3-13692) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Applications for Apolipoprotein E/ApoE Antibody (GT27711) - Azide and BSA Free

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Sandwich ELISA

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS, 20% Glycerol

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Apolipoprotein E/ApoE

Apolipoprotein E (APOE) is an apolipoprotein located in chylomicrons and intermediate-density lipoproteins that is necessary for the normal catabolism of triglyceride-rich lipoprotein constituents in the liver. APOE has recently been shown to be involseveral biological processes other than lipoprotein transport, including cognition and immunoregulation.

Lipoproteins are responsible for carrying cholesterol and other fats through the bloodstream as little packages and are essential for the normal breakdown of these molecules. In particular, apolipoprotein E is a major component of specific lipoproteins called very low-density lipoproteins (VLDL). A major function of VLDLs is to remove excess cholesterol from the blood and carry it to the liver for processing. Maintaining normal levels of cholesterol is essential for the prevention of cardiovascular diseases, including heart attacks and strokes.

The three Apo E isoforms are strongly associated with cardiovascular disease and Alzheimer's disease, so ApoE antibodies have become useful tools for research in both of these areas.

Alternate Names

APOE

Gene Symbol

APOE

Additional Apolipoprotein E/ApoE Products

Product Documents for Apolipoprotein E/ApoE Antibody (GT27711) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Apolipoprotein E/ApoE Antibody (GT27711) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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FAQs

No product specific FAQs exist for this product.

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Associated Pathways

A-beta Pathways: Uptake & Degradation
A-beta Pathways: Uptake & Degradation Thumbnail