Aquaporin-7 Antibody

Novus Biologicals | Catalog # NBP1-30862

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Porcine, Equine

Cited:

Mouse, Porcine, Bovine, Equine

Applications

Validated:

Immunohistochemistry-Frozen, Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Immunohistochemistry-Frozen, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
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Product Specifications

Immunogen

A synthetic peptide from amino acid region 2-50 of human Aquaporin-7 conjugated to blue carrier protein was used as the antigen.

Reactivity Notes

Equine reactivity reported in scientific literature (PMID: 29052325). Porcine reactivity reported in scientific literature (PMID: 28941027). Mouse reactivity reported in scientific literature (PMID: 31944177).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Aquaporin-7 Antibody

Aquaporin-7 Antibody

Immunocytochemistry/ Immunofluorescence: Aquaporin-7 Antibody [NBP1-30862] -

Exogenous expression of EGFP and EGFP+AQP7 cRNA in bovine MII oocytes. (A) Immunofluorescence analysis of AQP7 expression in bovine oocytes of non-injected, EGFP cRNA-injected and EGFP+AQP7 cRNA-injected oocytes. Nuclei counterstained with DAPI are displayed in blue (a1–a3), EGFP expression is displayed in green (b1–b3) and AQP7 protein expression is displayed in red (c1–c3). Overlayed images are shown in (d1–d3). White asterisk indicates chromosomes; white arrowhead indicates polar body. Scale bar, 30 μm. (B) Summary data of the relative immunofluorescence intensity normalized to control. Data are presented in arbitrary units +/- SEM, n ≥ 20, a,b Different letters indicate statistically significant differences between treatments (p < 0.05). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35203238), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
Aquaporin-7 Antibody

Immunocytochemistry/ Immunofluorescence: Aquaporin-7 Antibody [NBP1-30862] -

Effect of the exposure of IVM bovine oocytes to hyperosmotic CPA solutions on the levels of expression of AQP3 and AQP7. (A) Immunofluorescence analysis of AQP3 and AQP7 expression in bovine oocytes without treatment (a1 and a2, respectively) and in the presence of 9.5% Me2SO (b1 and b2, respectively), 8% EG (c1 and c2, respectively) or 0.5 M sucrose (d1 and d2, respectively). The concentrations of the two penetrating cryoprotectants, EG and Me2SO, were chosen to prepare solutions with similar osmolalities, as previously indicated [15]. AQP3 and AQP7 protein expression is displayed in green. Scale bar, 30 μm. (B) Summary data of the relative immunofluorescence intensity normalized to control. Data are presented in arbitrary units +/- SEM, n ≥ 25, a,b Different letters indicate statistically significant differences between treatments (p < 0.05). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35203238), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for Aquaporin-7 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

Reactivity reported in (PMID: 31944177)

Immunohistochemistry-Frozen

Reactivity reported in (PMID: 23628076)

Western Blot

1:1000

Formulation, Preparation, and Storage

Purification

Unpurified

Reconstitution

Reconstitute in 0.1 ml of sterile water. Centrifuge to remove any insoluble material. Glycerol may be added (1:1) for additional stability. Please note the sample size is provided in reconstituted format.

Formulation

Lyophilized from whole antisera

Preservative

No Preservative

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Aquaporin-7

Aquaporins/major intrinsic protein (MIP) are a family of water-selective membrane channels. Aquaporin 7 has greater sequence similarity with AQP3 and AQP9 and they may be a subfamily. Aquaporin 7 and AQP3 are at the same chromosomal location suggesting that 9p13 may be a site of an aquaporin cluster. Aquaporin 7 facilitates water, glycerol and urea transport. It may play an important role in sperm function.

Alternate Names

AQP-7, AQP7L, AQP9MGC149556, AQPapaquaglyceroporin-7, Aquaglyceroporin-7, aquaporin 7, Aquaporin adipose, aquaporin-7, Aquaporin-7-like, MGC149555

Entrez Gene IDs

364 (Human)

Gene Symbol

AQP7

UniProt

Additional Aquaporin-7 Products

Product Documents for Aquaporin-7 Antibody

Certificate of Analysis

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Product Specific Notices for Aquaporin-7 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Aquaporin-7 Antibody

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Protocols

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