The mouse ASAH2 gene encodes acylsphingosine amidohydrolase-2, a type II integral membrane protein that can be cleaved to produce a soluble secreted protein. The enzyme is abundant in intestinal brush border membranes, but is also expressed in the kidney, brain and liver. ASAH2 is involved in the metabolism of dietary sphingolipids and the generation of messenger molecules such as sphingosine and sphingosine 1-phosphate.
ASAH2/N-acylsphingosine Amidohydrolase-2 Antibody [CoraFluor™ 1]
Novus Biologicals | Catalog # NBP1-76360CL1
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Western Blot, ELISA
Label
CoraFluor 1
Antibody Source
Polyclonal Rabbit IgG
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Product Specifications
Immunogen
Antibody was raised against a 17 amino acid synthetic peptide near the carboxy terminus of the human ASAH2. The immunogen is located within the last 50 amino acids of ASAH2.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
This conjugate is made on demand and is not held in inventory. Each order represents a unique lot. All conjugates are generated using validated conjugation protocols and meet strict degree of labeling (F/P) specifications; additional information is available upon request. The antibody concentration is provided on the individual product label.
For specialized conjugation needs, like large quantities, specific concentrations, and defined F/P ratios, bulk and custom antibody conjugation services are available.
For specialized conjugation needs, like large quantities, specific concentrations, and defined F/P ratios, bulk and custom antibody conjugation services are available.
Scientific Data Images for ASAH2/N-acylsphingosine Amidohydrolase-2 Antibody [CoraFluor™ 1]
![ASAH2/N-acylsphingosine Amidohydrolase-2 Antibody [CoraFluor™ 1]](https://resources.rndsystems.com/images/products/nbp1-76360cl1_rabbit-asah2-n-acylsphingosine-amidohydrolase-2-pab-cl1-89202512233011.png "Product Feature: CoraFluor Probes for TR-FRET")
Product Feature: CoraFluor Probes for TR-FRET
CoraFluor™ 1, amine reactive (Catalog:7920) and CoraFluor™ 2, amine reactive (Catalog # 7950) are terbium-based probes that have been developed for use as TR-FRET donors. They emit wavelengths compatible with commonly used fluorescent acceptor dyes such as BODIPY® (or BDY) and Janelia Fluor® dyes, FITC (Catalog # 5440), TMR and Cyanine 5 (Catalog # 5436). CoraFluor™ fluorescence is brighter and more stable in biological media than existing TR-FRET donors, leading to enhanced sensitivity and improved data generation. CoraFluor™ 1 exhibits excitation upon exposure to a 337 nm UV laser.Applications for ASAH2/N-acylsphingosine Amidohydrolase-2 Antibody [CoraFluor™ 1]
Application
Recommended Usage
ELISA
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Recommended applications are based on validated applications from the unconjugated base product NBP1-76360. This conjugated antibody is not kept in inventory and is made to order using validated conjugation protocols.
Formulation, Preparation, and Storage
Purification
Peptide affinity purified
Formulation
PBS
Preservative
No Preservative
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark. Do not freeze.
Background: ASAH2/N-acylsphingosine Amidohydrolase-2
Long Name
Neutral Ceramidase
Alternate Names
Nacylsphingosine Amidohydrolase2
Gene Symbol
ASAH2
Additional ASAH2/N-acylsphingosine Amidohydrolase-2 Products
Product Documents for ASAH2/N-acylsphingosine Amidohydrolase-2 Antibody [CoraFluor™ 1]
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for ASAH2/N-acylsphingosine Amidohydrolase-2 Antibody [CoraFluor™ 1]
CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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Protocols
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