ASCL1/Mash1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-99418

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Mouse

Predicted:

Rat (100%). Backed by our 100% Guarantee.

Applications

Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all ASCL1/Mash1 Primary Antibodies »

Product Specifications

Immunogen

Produced in rabbits immunized with a synthetic peptide corresponding to the center region of the Human ASCL1/Mash1

Specificity

Mouse MASH1/ASCL1

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for ASCL1/Mash1 Antibody - BSA Free

Western Blot: ASCL1/Mash1 Antibody [NBP2-99418]

Western Blot: ASCL1/Mash1 Antibody [NBP2-99418]

Western Blot: ASCL1/Mash1 Antibody [NBP2-99418] - Anti-ASCL1/Mash1 rabbit polyclonal antibody at 1:500 dilution. Lane A: Mouse brain tissue lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 25 kDa. Observed band size: 28 kDa
Immunoprecipitation: ASCL1/Mash1 Antibody [NBP2-99418]

Immunoprecipitation: ASCL1/Mash1 Antibody [NBP2-99418]

Immunoprecipitation: ASCL1/Mash1 Antibody [NBP2-99418] - ASCL1/Mash1 was immunoprecipitated using: Lane A: 0.5 mg Mouse brains Tissue Lysate 4 ul anti-ASCL1/Mash1 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads. Protein A/G. Primary antibody: Anti-ASCL1/Mash1 rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 25 kDa. Observed band size: 29 kDa

Applications for ASCL1/Mash1 Antibody - BSA Free

Application
Recommended Usage

Immunoprecipitation

1-5 uL/mg of lysate

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

PBS

Format

BSA Free

Preservative

0.03% Proclin 300

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ASCL1/Mash1

MASH1 encodes a member of the basic helix-loop-helix (BHLH) family of transcription factors. The protein activates transcription by binding to the E box (5'-CANNTG-3'). Dimerization with other BHLH proteins is required for efficient DNA binding. This protein plays a role in the neuronal commitment and differentiation and in the generation of olfactory and autonomic neurons. Mutations in this gene may contribute to the congenital central hypoventilation syndrome (CCHS) phenotype in rare cases.

Long Name

Achaete Scute Complex-like 1

Alternate Names

ASCL1, ASH1, HASH1, Mash1

Gene Symbol

ASCL1

Additional ASCL1/Mash1 Products

Product Documents for ASCL1/Mash1 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for ASCL1/Mash1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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