ATF6 Antibody - BSA Free
Novus Biologicals | Catalog # NBP1-77251
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Scientific Data Images for ATF6 Antibody - BSA Free
Immunohistochemistry: ATF6 Antibody - BSA Free [NBP1-77251] -
Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-ATF6 antibody at 5 ug/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
Western Blot: ATF6 Antibody - BSA Free [NBP1-77251] -
Loading: 15 ug of rat kidney lysate Antibodies: ATF6, 1 ug/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.
Western Blot: ATF6 Antibody - BSA Free [NBP1-77251] -
Loading: 15 ug of mouse tissue lysate Antibodies: ATF6, 1 ug/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.
Immunohistochemistry: ATF6 Antibody - BSA Free [NBP1-77251] -
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-ATF6 antibody at 5 ug/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
Western Blot: ATF6 Antibody - BSA Free [NBP1-77251] -
Loading: 15 ug of human cell lysate Antibodies ATF6, 1 ug/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.conjugate at 1:10000 dilution.Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.conjugate at 1:10000 dilution.
Immunohistochemistry: ATF6 Antibody - BSA Free [NBP1-77251] -
Immunohistochemical analysis of paraffin-embedded human testis tissue using anti-ATF6 antibody at 5 ug/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.Applications for ATF6 Antibody - BSA Free
Immunohistochemistry
Western Blot
Formulation, Preparation, and Storage
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Format
Preservative
Concentration
Shipping
Stability & Storage
Background: ATF6
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UniProt
Additional ATF6 Products
Product Documents for ATF6 Antibody - BSA Free
Certificate of Analysis
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Product Specific Notices for ATF6 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Citations for ATF6 Antibody - BSA Free
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- R&D Systems Quality Control Western Blot Protocol
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
FAQs for ATF6 Antibody - BSA Free
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Q: May we ask if ATF6 is ATF6 alpha? We are looking for ATF6 alpha antibody for our customer, but found none from Novus. Only ATF6 antibodies. However, after checking http://www.uniprot.org/uniprot/P18850, I found ATF6 and ATF6 alpha seem identical. Would you please help confirm if NBP1-40256, NBP1-77251, NBP1-76675 & NBP1-41439 are all ATF6 alpha antibodies?
A: The antibodies NBP1-77251, NBP1-76675 are specific to ATF6A and should not cross-react with ATF6B.
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Q: We are looking for an antibody specific to ATF6 alpha that will not cross-react with ATF6 beta. Would you please help confirm if any of the following are ATF6 alpha specific antibodies: NBP1-40256, NBP1-77251, NBP1-76675 & NBP1-41439?
A:
The antibodies NBP1-77251 and NBP1-76675 are specific to ATF6 alpha and should not cross-react with ATF6 beta.
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Q: May we ask if ATF6 is ATF6 alpha? We are looking for ATF6 alpha antibody for our customer, but found none from Novus. Only ATF6 antibodies. However, after checking http://www.uniprot.org/uniprot/P18850, I found ATF6 and ATF6 alpha seem identical. Would you please help confirm if NBP1-40256, NBP1-77251, NBP1-76675 & NBP1-41439 are all ATF6 alpha antibodies?
A: The antibodies NBP1-77251, NBP1-76675 are specific to ATF6A and should not cross-react with ATF6B.
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Q: We are looking for an antibody specific to ATF6 alpha that will not cross-react with ATF6 beta. Would you please help confirm if any of the following are ATF6 alpha specific antibodies: NBP1-40256, NBP1-77251, NBP1-76675 & NBP1-41439?
A:
The antibodies NBP1-77251 and NBP1-76675 are specific to ATF6 alpha and should not cross-react with ATF6 beta.