ATG12 Antibody - BSA Free

Novus Biologicals | Catalog # NB600-603

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Yeast

Cited:

Bacteria

Applications

Validated:

Western Blot, ELISA

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all ATG12 Primary Antibodies »

Product Specifications

Immunogen

This purified ATG12 Antibody was prepared from rabbit serum after repeated immunizations with recombinant yeast ATG12 protein. (Uniprot: P38316)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum.

Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

Scientific Data Images for ATG12 Antibody - BSA Free

Western Blot: ATG12 Antibody [NB600-603]

Western Blot: ATG12 Antibody [NB600-603]

Western Blot: ATG12 Antibody [NB600-603] - Immunoblot of APG12 fusion protein. Anti-APG12 antibody generated by immunization with recombinant yeast APG12 was tested by immunoblot against yeast lysates expressing the APG12-GFP fusion protein and other UBL fusion proteins. All UBLs possess limited homology to Ubiquitin and to each other, therefore it is important to know the degree of reactivity of each antibody against each UBL.
N/A: ATG12 Antibody [NB600-603]

Applications for ATG12 Antibody - BSA Free

Application
Recommended Usage

ELISA

1:1000-1:5000

Western Blot

1:500-1:2000
Application Notes
This purified polyclonal antibody reacts yeast APG12 by western blot and ELISA. Although not tested, this antibody is likely functional in immunohistochemistry and immunoprecipitation. This antibody using the specified conditions may recognize other prominent intrinsic bands (UBLs or their conjugates). Other intrinsic bands are readily detectable in yeast lysates at lower antibody dilutions.

Reviewed Applications

Read 1 review rated 1 using NB600-603 in the following applications:

Formulation, Preparation, and Storage

Purification

Multi-step

Formulation

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Format

BSA Free

Preservative

0.01% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: ATG12

Ubiquitin-like proteins fall into two classes: the first class, ubiquitin-like modifiers (UBLs) function as modifiers in a manner analogous to that of ubiquitin. Examples of UBLs are SUMO, Rub1 (also called Nedd8), Apg8 and Apg12. Proteins of the second class include parkin, RAD23 and DSK2, are designated ubiquitin-domain proteins (UDPs). These proteins contain domains that are related to ubiquitin but are otherwise unrelated to each other. In contrast to UBLs, UDPs are not conjugated to other proteins. In yeast, autophagy, the delivery of cytoplasmic components to the lysosome/vacuole for degradation, requires a ubiquitin-like protein conjugation system, in which Apg12 is covalently bound to Apg12-Apg5 and Apg16.

Long Name

ATG12 Autophagy Related 12 Homolog

Alternate Names

APG12, FBR93, HAPG12

Gene Symbol

ATG12

UniProt

P38316 (Yeast)

Additional ATG12 Products

Product Documents for ATG12 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for ATG12 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Autophagy

Citations for ATG12 Antibody - BSA Free

Customer Reviews for ATG12 Antibody - BSA Free (1)

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  • ATG12 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Total protein lysate (TCA precipitated) and Total yeast protein (TCA precipitated)
    Species: S. Cerevisiae
    Verified Customer | Posted 06/01/2022
    Native Atg12 could not be detected in yeast cell lysates. Expect ~63 kDa in WT lanes and ~25 kDa in the atg10D lane, but only nonspecific bands, also present in the atg12D lanes, could be seen.
    Incubated overnight 4C; secondary anti-rabbit (1:5000) incubated 1 hour RT, all in 4% milk/TBST blocking solution.
    ATG12 Antibody - BSA Free NB600-603
    Bio-Techne Response
    This review was submitted through the legacy Novus Innovators Program, reflecting a new species or application tested on a primary antibody.

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