ATG4B Antibody (PSH02-53)

Novus Biologicals | Catalog # NBP3-32058

Recombinant Monoclonal Antibody
Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat, Monkey

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # PSH02-53 expressed in HEK293
View all ATG4B Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein within human ATG4B aa 1-393 / 393 (Q9Y4P1). (Uniprot: Q9Y4P1)

Localization

Cytoplasm, cytosol, Cytoplasmic vesicle, autophagosome, Endoplasmic reticulum, Mitochondrion.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

44 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ATG4B Antibody (PSH02-53)

ATG4B Antibody (PSH02-53) Western Blot: ATG4B Antibody (PSH02-53) [NBP3-32058]

Western Blot: ATG4B Antibody (PSH02-53) [NBP3-32058]

Western blot analysis on different lysates with NBP3-32058 at 1/2,000 dilution and competitor's antibody at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: Jurkat cell lysate Lane 3: 293T cell lysate Lane 4: MCF7 cell lysate Lane 5: K-562 cell lysate Lane 6: COS-1 cell lysate Lane 7: NIH/3T3 cell lysate Lane 8: C2C12 cell lysate Lane 9: PC-12 cell lysate Lane 10: Mouse brain tissue lysate Lane 11: Rat brain tissue lysate Lane 12: Mouse kidney tissue lysate Lane 13: Mouse colon tissue lysate Lysates/proteins at 30 µg/Lane. Predicted band size: 44 kDa Observed band size: 47 kDa Exposure time: 42 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP3-32058 at 1/2,000 dilution and competitor's antibody at 1/2,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG [HRP] Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
ATG4B Antibody (PSH02-53) Western Blot: ATG4B Antibody (PSH02-53) [NBP3-32058]

Western Blot: ATG4B Antibody (PSH02-53) [NBP3-32058]

Western blot analysis on different lysates with NBP3-32058 at 1/2,000 dilution. Lane 1: 293T cell lysate (20 µg/Lane) Lane 2: MCF7 cell lysate (20 µg/Lane) Lane 3: COS-1 cell lysate (20 µg/Lane) Lane 4: NIH/3T3 cell lysate (20 µg/Lane) Lane 5: PC-12 cell lysate (20 µg/Lane) Lane 6: Mouse brain tissue lysate (40 µg/Lane) Lane 7: Rat brain tissue lysate (40 µg/Lane) Predicted band size: 44 kDa Observed band size: 47 kDa Exposure time: 20 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP3-32058 at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG [HRP] Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.

Applications for ATG4B Antibody (PSH02-53)

Application
Recommended Usage

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH7.4), 0.1% BSA and 40% Glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ATG4B

FUNCTION: Cysteine protease required for autophagy, which cleaves the C-terminal part of either MAP1LC3, GABARAPL2 or GABARAP, allowing the liberation of form I. A subpopulation of form I is subsequently converted to a smaller form (form II). Form II, with a revealed C-terminal glycine, is considered to be the phosphatidylethanolamine (PE)-conjugated form, and has the capacity for the binding to autophagosomes. ENZYME REGULATION: Inhibited by N-ethylmaleimide. SUBCELLULAR LOCATION: Cytoplasm (Probable). ALTERNATIVE PRODUCTS: 5 named isoforms produced by alternative splicing.

Long Name

ATG4 Autophagy Related 4 Homolog B

Alternate Names

Apg4B, AUTL1

Gene Symbol

ATG4B

Additional ATG4B Products

Product Documents for ATG4B Antibody (PSH02-53)

Certificate of Analysis

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Product Specific Notices for ATG4B Antibody (PSH02-53)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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