ATG5 Antibody (CL9508) - BSA Free

Novus Biologicals | Catalog # NBP2-88936

Novus Biologicals
100% Guarantee

Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Predicted:

Mouse (96%), Rat (94%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL9508

Format

BSA Free
View all ATG5 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed using a recombinant protein derived from Q9H1Y0, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for ATG5 Antibody (CL9508) - BSA Free

Immunohistochemistry-Paraffin: ATG5 Antibody (CL9508) [NBP2-88936]

Immunohistochemistry-Paraffin: ATG5 Antibody (CL9508) [NBP2-88936]

Immunohistochemistry-Paraffin: ATG5 Antibody (CL9508) [NBP2-88936] - Immunohistochemistry analysis in human prostate and skeletal muscle tissues using NBP2-88936 antibody. Corresponding ATG5 RNA-seq data are presented for the same tissues.
Western Blot: ATG5 Antibody (CL9508) [NBP2-88936]

Western Blot: ATG5 Antibody (CL9508) [NBP2-88936]

Western Blot: ATG5 Antibody (CL9508) [NBP2-88936] - Western blot analysis in human cell line RH-30.
Immunohistochemistry-Paraffin: ATG5 Antibody (CL9508) [NBP2-88936]

Immunohistochemistry-Paraffin: ATG5 Antibody (CL9508) [NBP2-88936]

Immunohistochemistry-Paraffin: ATG5 Antibody (CL9508) [NBP2-88936] - Immunohistochemistry analysis of human endometrium shows moderate cytoplasmic positivity in glandular cells.
Immunohistochemistry-Paraffin: ATG5 Antibody (CL9508) [NBP2-88936]

Immunohistochemistry-Paraffin: ATG5 Antibody (CL9508) [NBP2-88936]

Immunohistochemistry-Paraffin: ATG5 Antibody (CL9508) [NBP2-88936] - Immunohistochemistry analysis of human prostate cancer shows moderate cytoplasmic positivity in glandular cells.
Immunohistochemistry-Paraffin: ATG5 Antibody (CL9508) [NBP2-88936]

Immunohistochemistry-Paraffin: ATG5 Antibody (CL9508) [NBP2-88936]

Immunohistochemistry-Paraffin: ATG5 Antibody (CL9508) [NBP2-88936] - Immunohistochemistry analysis of human prostate shows strong cytoplasmic positivity in glandular cells.
Immunohistochemistry-Paraffin: ATG5 Antibody (CL9508) [NBP2-88936]

Immunohistochemistry-Paraffin: ATG5 Antibody (CL9508) [NBP2-88936]

Immunohistochemistry-Paraffin: ATG5 Antibody (CL9508) [NBP2-88936] - Immunohistochemistry analysis of human skeletal muscle shows no positivity in myocytes as expected.
Immunohistochemistry-Paraffin: ATG5 Antibody (CL9508) [NBP2-88936]

Immunohistochemistry-Paraffin: ATG5 Antibody (CL9508) [NBP2-88936]

Immunohistochemistry-Paraffin: ATG5 Antibody (CL9508) [NBP2-88936] - Immunohistochemistry analysis of human testis shows moderate to strong cytoplasmic positivity in cells in seminiferous ducts.

Applications for ATG5 Antibody (CL9508) - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000

Western Blot

1 ug/mL
Application Notes
For IHC-Paraffin, HIER pH 6 antigen retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ATG5

Atg5, Autophagy related 5 or autophagy protein 5 (theoretical molecular weight 32 kDa), belongs to a group of core autophagy-related proteins first identified in yeast and later in eukaryotic cells. Atg proteins play essential roles in the process of macroautophagy. Atg5 is considered a core autophagy protein, for its role in the formation of the autophagosome, a double membrane vesicle which engulfs proteins and organelles for delivery to the lysosome and subsequent degradation (1). Atg5 participates in the process of phagophore elongation by interacting with the ubiquitin-like protein Atg12. Formation of the Atg12-Atg5 conjugate is dependent on the activities of Atg7 (E1 ubiquitin-activating like enzyme) and Atg10 (E2 ubiquitin-activating like enzyme). Non-covalent interaction between the Atg12-Atg5 conjugate and Atg16L1, allows for the formation of a large complex which associates with the nascent phagophore. The Atg16L1 complex dissociates from the autophagosome once it is fully formed (1,2).

In the context of its role in autophagy, Atg5 plays diverse physiologically relevant roles. For example, Atg5 together with Atg7 are required for adipogenesis (3). Recently, Atg5 has been implicated in the process of B-cell receptor polarization and antigen presentation (4). In addition to its role in autophagy, Atg5 is implicated in apoptotic cell death. Interaction of Atg5 with FADD (Fas-associated protein with death domain) is involved in cell death induced by IFN-gamma. A truncated form of Atg5, a 24kDa fragment, leads to cell death by interacting with Bcl-xl and inhibiting its anti-apoptotic activity (5). Other Atg5 interacting partners include interleukin-beta (IL-beta) converting enzyme and nucleotide binding oligomerization domain protein 1, which suggest that Atg5 may play other biologically relevant roles (3).

References

1. Yang, Z., & Klionsky, D. J. (2010). Mammalian autophagy: Core molecular machinery and signaling regulation. Current Opinion in Cell Biology. https://doi.org/10.1016/j.ceb.2009.11.014

2. Rubinsztein, D. C., Shpilka, T., & Elazar, Z. (2012). Mechanisms of autophagosome biogenesis. Current Biology. https://doi.org/10.1016/j.cub.2011.11.034

3. Subramani, S., & Malhotra, V. (2013). Non-autophagic roles of autophagy-related proteins. EMBO Reports. https://doi.org/10.1038/embor.2012.220

4. Arbogast, F., Arnold, J., Hammann, P., Kuhn, L., Chicher, J., Murera, D., Gros, F. (2019). ATG5 is required for B cell polarization and presentation of particulate antigens. Autophagy. https://doi.org/10.1080/15548627.2018.1516327

5. Luo, S., & Rubinsztein, D. C. (2007). Atg5 and Bcl-2 provide novel insights into the interplay between apoptosis and autophagy. Cell Death and Differentiation. https://doi.org/10.1038/sj.cdd.4402149

Long Name

ATG5 Autophagy Related 5 Homolog

Alternate Names

APG5, ASP

Gene Symbol

ATG5

Additional ATG5 Products

Product Documents for ATG5 Antibody (CL9508) - BSA Free

Certificate of Analysis

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Product Specific Notices for ATG5 Antibody (CL9508) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Autophagy

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Protocols

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