ATIC Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-32183

Novus Biologicals
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse

Predicted:

Chicken (100%), Orangutan (100%). Backed by our 100% Guarantee.

Applications

Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all ATIC Primary Antibodies »

Product Specifications

Immunogen

The immunogen this antibody was made to, maps to a region between residue 50 to 100 of human 5-aminoimidazole-4-carboxamide Ribonucleotide Formyltransferase/IMP Cyclohydrolase using the numbering given in entry NP_004035.2 (GeneID 471).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ATIC Antibody - BSA Free

Western Blot: ATIC Antibody [NBP2-32183]

Western Blot: ATIC Antibody [NBP2-32183]

Western Blot: ATIC Antibody [NBP2-32183] - Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells. Antibodies: Affinity purified rabbit anti-ATIC antibody NBP2-32183 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Immunoprecipitation: ATIC Antibody [NBP2-32183]

Immunoprecipitation: ATIC Antibody [NBP2-32183]

Immunoprecipitation: ATIC Antibody [NBP2-32183] - Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells. Antibodies: Affinity purified rabbit anti-ATIC antibody NBP2-32183 used for IP at 6 ug per reaction. For blotting immunoprecipitated ATIC, NBP2-32183 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.

Applications for ATIC Antibody - BSA Free

Application
Recommended Usage

Immunoprecipitation

2 - 10 ug/mg lysate

Western Blot

1:2000 - 1:10000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: ATIC

ATIC is a 5 aminoimidazole 4 carboxamide ribonucleotide formyltransferase which helps catalyze the penultimate and final steps of the de novo purine biosynthetic pathway. The human ATIC cDNA encodes a deduced 591 amino acid protein that is 81% identical to the chicken sequence.

Alternate Names

5-aminoimidazole-4-carboxamide ribonucleotide formyltransferase/IMPcyclohydrolase, 5-aminoimidazole-4-carboxamide-1-beta-D-ribonucleotidetransformylase/inosinicase, AICAR, AICAR formyltransferase/IMP cyclohydrolase bifunctional enzyme, AICARFT, AICARFT/IMPCHASE, bifunctional purine biosynthesis protein PURH, IMPCHASE, phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase, PURHFLJ93545

Gene Symbol

ATIC

UniProt

NP_004035.2 (Human)

Additional ATIC Products

Product Documents for ATIC Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for ATIC Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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