Aurora B Antibody (6G2) - BSA Free

Novus Biologicals | Catalog # NBP2-50054

Novus Biologicals
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Human, Canine, Equine

Applications

Immunohistochemistry, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 6G2

Format

BSA Free
View all Aurora B Primary Antibodies »

Product Specifications

Immunogen

Recombinant full length human Aurora B. [UniProt# Q96GD4]

Specificity

This antibody does not cross-react with Aurora A or Aurora C.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

38 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Aurora B Antibody (6G2) - BSA Free

Western Blot: Aurora B Antibody (6G2) [NBP2-50054]

Western Blot: Aurora B Antibody (6G2) [NBP2-50054]

Western Blot: Aurora B Antibody (6G2) [NBP2-50054] - Left: Analysis of HeLa cells treated with 100ng/ml nocodazole for 18 hours was probed with NBP2-50054. Nocodazole is a microtubule polymerization inhibitor which induces cells to halt at the G2/M phase and also induces Aurora B expression. NBP2-50054 binds strongly to Aurora B at 38 kDa. Right: Blots of recombinant human Aurora A, B and C were probed with NBP2-50054.
Immunocytochemistry/ Immunofluorescence: Aurora B Antibody (6G2) [NBP2-50054]

Immunocytochemistry/ Immunofluorescence: Aurora B Antibody (6G2) [NBP2-50054]

Immunocytochemistry/Immunofluorescence: Aurora B Antibody (6G2) [NBP2-50054] - Analysis of HeLa cells stained with mouse mAb to aurora B kinase, NBP2-50054, dilution 1:1,000 in red, and costained with chicken pAb to vimentin, dilution 1:10,000 in green. Blue is DAPI staining of nuclear DNA. NBP2-50054 antibody produces strong staining associated with chromosomes in prophase (A), the centromere in prometaphase and metaphase (B), the central mitotic spindle in anaphase (C), and midbodies between the two daughter cells during telophase and beyond (D). The vimentin antibody stains the intermediate filament network in these cells.
Immunocytochemistry/ Immunofluorescence: Aurora B Antibody (6G2) [NBP2-50054]

Immunocytochemistry/ Immunofluorescence: Aurora B Antibody (6G2) [NBP2-50054]

Immunocytochemistry/Immunofluorescence: Aurora B Antibody (6G2) [NBP2-50054] - HeLa cell cultures were stained with NBP2-50054 (green). Localization of Aurora B is cell cycle phase dependent. First, Aurora B staining was seen in chromosome arm in cells in prophase (green small dots). During anaphase, Aurora B localized in midzone and then concentrated in midbodies between the two daughter cells during telophase. It is therefore a useful marker of midbodies and dividing cells. Cells were counterstained with chicken polyclonal antibody to Vimentin (NB300-223, red). Blue is a DNA stain.

Applications for Aurora B Antibody (6G2) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:1000 - 1:2000

Immunohistochemistry

1:1000 - 1:2000

Western Blot

1:1000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

50% PBS, 50% glycerol

Format

BSA Free

Preservative

0.035% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Aurora B

Aurora-B is a member of a novel family of serine/threonine kinases that have been identified as key regulators of the mitotic cell division process. Aurora-B is known to be involved in the regulation of centrosome function, bipolar spindle assembly and chromosome segregation. Aurora kinases are localized at the centrosomes of interphase cells, at the poles of the bipolar spindle and in the midbody of the mitotic apparatus. Substrates identified for the Aurora-B kinases include a kinesin-like motor protein, spindle apparatus proteins, histone H3 protein, kinetochore protein and the tumor suppressor protein p53 (1). Overexpression of aurora B produced multinuclearity and induced aggressive metastasis, suggesting that overexpressed aurora B has multiple functions in cancer development. Identification of Aurora kinases as RasGAP Src homology 3 domain binding protein, also implicates these kinases as potential effectors in the Ras pathway relevant to oncogenesis (2).

Alternate Names

Aik2, AIM-1, AIM1, ARK2, AURKB, IPL1, STK12, STK5

Gene Symbol

AURKB

UniProt

Q96GD4

Additional Aurora B Products

Product Documents for Aurora B Antibody (6G2) - BSA Free

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Product Specific Notices for Aurora B Antibody (6G2) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Cell Cycle
Epigenetics
Microtubules

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Protocols

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