bcl-x Antibody (BCL2L1/1762) - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-08580

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

ELISA

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # BCL2L1/1762

Format

Azide and BSA Free
View all Bcl-x Primary Antibodies »

Product Specifications

Immunogen

Recombinant human bcl-x protein fragment (around aa 1-135) (exact sequence is proprietary) (Uniprot: Q07817)

Localization

Cytoplasmic and cell / nuclear membrane

Marker

Apoptosis Marker

Specificity

Recognizes a protein of 27kDa, identified as the Bcl-X protein. This monoclonal antibody shows no cross-reaction with Bcl-2 or Bax protein. Bcl-X has two isoforms, Bcl-XL (long), a 241-amino acid protein which suppresses cell death. And Bcl-XS (short), a 178-amino acid protein lacking a 63-amino acid domain which functions as a dominant inhibitor of Bcl-2.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Theoretical MW

27 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide.

Antibody without azide - store at -20 to -80C.

Scientific Data Images for bcl-x Antibody (BCL2L1/1762) - Azide and BSA Free

bcl-x Antibody (BCL2L1/1762) - Azide and BSA Free Western Blot: bcl-x Antibody (BCL2L1/1762) - Azide and BSA Free [NBP3-08580] -

Western Blot: bcl-x Antibody (BCL2L1/1762) - Azide and BSA Free [NBP3-08580] -

Western Blot Analysis of HeLa, Jurkat, NIH/3T3, Hep G2 and HDLM-2 cell lysates using bcl-x Antibody (BCL2L1/1762) - Azide and BSA Free.

Applications for bcl-x Antibody (BCL2L1/1762) - Azide and BSA Free

Application
Recommended Usage

ELISA

1-5 ug/ml
Application Notes
ELISA: 1-5ug/ml. Order antibody without BSA. Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: Bcl-x

Bcl-x is a member of the Bcl-2 family of proteins that regulates outer mitochondrial membrane permeability. Alternative splicing results in two distinct Bcl-x isoforms. Bcl-xL (long) is an anti-apoptotic protein that prevents release of Cytochromec from the mitochondrial intermembrane space into the cytosol. Bcl-xs (short) is a pro-apoptotic member that can initiate programmed cell death.

Long Name

B Cell Lymphoma/Leukemia x

Alternate Names

BCL2L1, Bclx

Gene Symbol

BCL2L1

Additional Bcl-x Products

Product Documents for bcl-x Antibody (BCL2L1/1762) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for bcl-x Antibody (BCL2L1/1762) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Bcl-2 Proteins and Regulators

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