beta-Catenin Antibody (CL3689) - BSA Free

Name: beta-Catenin Antibody (CL3689) - BSA Free
Brand: Novus Biologicals
SKU: NBP2-61628
Price: 269 USD
Availability: InStock

Novus Biologicals | Catalog # NBP2-61628

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG_2A Clone # CL3689

Format

BSA Free

View all beta-Catenin Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed using a recombinant protein derived from P35222, with the exact immunogen sequence remaining proprietary.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions

Clonality

Monoclonal

Host

Mouse

Isotype

IgG_2A

Scientific Data Images for beta-Catenin Antibody (CL3689) - BSA Free

Western Blot: beta-Catenin Antibody (CL3689) [NBP2-61628]

Western Blot: beta-Catenin Antibody (CL3689) [NBP2-61628] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10
Lane 2: Human cell line A-549

Immunocytochemistry/ Immunofluorescence: beta-Catenin Antibody (CL3689) [NBP2-61628]

Immunocytochemistry/Immunofluorescence: beta-Catenin Antibody (CL3689) [NBP2-61628] - Staining of A-431 cells using the Anti-CTNNB1 monoclonal antibody, showing specific staining in the plasma membrane in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).

Immunohistochemistry-Paraffin: beta-Catenin Antibody (CL3689) [NBP2-61628]

Immunohistochemistry-Paraffin: beta-Catenin Antibody (CL3689) [NBP2-61628] - Staining of human skeletal muscle shows absence of immunoreactivity in muscle fibers (negative control).

Western Blot: beta-Catenin Antibody (CL3689) [NBP2-61628]

Western Blot: beta-Catenin Antibody (CL3689) [NBP2-61628] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10
Lane 2: Human cell line SK-MEL-30

Immunocytochemistry/ Immunofluorescence: beta-Catenin Antibody (CL3689) [NBP2-61628]

Immunocytochemistry/Immunofluorescence: beta-Catenin Antibody (CL3689) [NBP2-61628] - Staining of U-251 cells using the Anti-CTNNB1 monoclonal antibody, showing specific staining in the plasma membrane in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).

Immunohistochemistry: beta-Catenin Antibody (CL3689) [NBP2-61628]

Immunohistochemistry: beta-Catenin Antibody (CL3689) [NBP2-61628] - Staining of human endometrium shows strong membranous immunoreactivity in glandular epithelium.

Immunohistochemistry: beta-Catenin Antibody (CL3689) [NBP2-61628]

Immunohistochemistry: beta-Catenin Antibody (CL3689) [NBP2-61628] - Staining of human kidney shows strong membranous positivity in renal tubules and glomerulus cells.

Immunohistochemistry: beta-Catenin Antibody (CL3689) [NBP2-61628]

Immunohistochemistry: beta-Catenin Antibody (CL3689) [NBP2-61628] - Staining of human duodenum shows strong membranous immunoreactivity in epithelial cells.

Immunohistochemistry: beta-Catenin Antibody (CL3689) [NBP2-61628]

Immunohistochemistry: beta-Catenin Antibody (CL3689) [NBP2-61628] - Staining of human cervix shows strong membranous positivity in epithelial cells.

Immunohistochemistry-Paraffin: beta-Catenin Antibody (CL3689) [NBP2-61628]

Immunohistochemistry-Paraffin: beta-Catenin Antibody (CL3689) [NBP2-61628] - Staining of human breast cancer shows membranous immunoreactivity in tumor cells.

Immunohistochemistry-Paraffin: beta-Catenin Antibody (CL3689) [NBP2-61628]

Immunohistochemistry-Paraffin: beta-Catenin Antibody (CL3689) [NBP2-61628] - Staining of human prostate cancer shows membranous positivity in tumor cells.

Immunohistochemistry-Paraffin: beta-Catenin Antibody (CL3689) [NBP2-61628]

Immunohistochemistry-Paraffin: beta-Catenin Antibody (CL3689) [NBP2-61628] - Staining of human renal cancer shows membranous positivity in tumor cells.

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Applications for beta-Catenin Antibody (CL3689) - BSA Free

Application

Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Immunohistochemistry

1:20000 - 1:50000

Immunohistochemistry-Paraffin

1:20000 - 1:50000

Western Blot

1 ug/ml

Application Notes

For IHC-Paraffin, HIER pH 6 retrieval is recommended. For ICC/IF, PFA/Triton X-100 is recommended for fixation/permeabilization.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: beta-Catenin

The protein encoded by the beta Catenin gene is part of a complex of proteins that constitute adherens junctions (AJs). AJs arenecessary for the creation and maintenance of epithelial cell layers by regulating cell growth and adhesion betweencells. The encoded protein also anchors the actin cytoskeleton and may be responsible for transmitting the contactinhibition signal that causes cells to stop dividing once the epithelial sheet is complete. Finally, this proteinbinds to the product of the APC gene, which is mutated in adenomatous polyposis of the colon. Mutations in this geneare a cause of colorectal cancer (CRC), pilomatrixoma (PTR), medulloblastoma (MDB), and ovarian cancer. Three transcript variants encoding the same protein have been found for this gene.

Alternate Names

bCatenin, CTNNB1

Gene Symbol

CTNNB1

Additional beta-Catenin Products

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Product Specific Notices for beta-Catenin Antibody (CL3689) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.