beta-Catenin Antibody (SA30-04)

Name: beta-Catenin Antibody (SA30-04)
Brand: Novus Biologicals
SKU: NBP2-67307
Price: 439 USD
Availability: InStock

Novus Biologicals | Catalog # NBP2-67307

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Product Documents

Recombinant Monoclonal Antibody

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Multiplex Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # SA30-04 expressed in HEK293

View all beta-Catenin Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptide within human beta-Catenin aa 30-70. (SwissProt: P35222 Human; SwissProt: Q02248 Mouse; SwissProt: Q9WU82 Rat)

Localization

Cytoplasm, Nucleus, Cell membrane, Cell junction

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for beta-Catenin Antibody (SA30-04)

Western Blot: beta-Catenin Antibody (SA30-04) [NBP2-67307]

Western Blot: beta-Catenin Antibody (SA30-04) [NBP2-67307] - Analysis of Beta-Catenin on HT-29 cell lysates with Rabbit anti-Beta-Catenin antibody at 1/500 dilution. Lysates/proteins at 10 ug/Lane. Predicted band size: 85 kDa Observed band size: 85 kDa Exposure time: 1 minute; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.

Immunocytochemistry: beta-Catenin Antibody (SA30-04) [NBP2-67307]

Immunocytochemistry: beta-Catenin Antibody (SA30-04) [NBP2-67307] - Usage of Beta Catenin antibody in ICC of HT-29 cells

Immunohistochemistry-Paraffin: beta-Catenin Antibody (SA30-04) [NBP2-67307]

Immunohistochemistry-Paraffin: beta-Catenin Antibody (SA30-04) [NBP2-67307] - Immunofluorescence analysis of paraffin-embedded human colon carcinoma tissue labeling Beta-Catenin with Rabbit anti-Beta-Catenin antibody washed with PBS, and then probed with the primary antibody (green) at 1/100 dilution overnight at 4, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor(TM) 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).

Western Blot: beta-Catenin Antibody (SA30-04) [NBP2-67307]

Western Blot: beta-Catenin Antibody (SA30-04) [NBP2-67307] - Analysis of Beta-Catenin on different cell lysates using anti- Beta-Catenin antibody at 1/1,000 dilution. Positive control: Lane 1: SW480 Lane 2: HT29 Lane 3: A431

Western Blot: beta-Catenin Antibody (SA30-04) [NBP2-67307]

Western Blot: beta-Catenin Antibody (SA30-04) [NBP2-67307] - Analysis of Beta-Catenin on different lysates with Rabbit anti-Beta-Catenin antibody at 1/500 dilution. Lane 1: SW480 cell lysate Lane 2: A431 cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 85 kDa Observed band size: 85 kDa Exposure time: 30 seconds; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.

Immunohistochemistry-Paraffin: beta-Catenin Antibody (SA30-04) [NBP2-67307]

Immunohistochemistry-Paraffin: beta-Catenin Antibody (SA30-04) [NBP2-67307] - Analysis of mouse small intestine tissue using anti-b-Catenin antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: beta-Catenin Antibody (SA30-04) [NBP2-67307]

Immunohistochemistry-Paraffin: beta-Catenin Antibody (SA30-04) [NBP2-67307] - Analysis of mouse colon tissue using anti- Beta-Catenin antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: beta-Catenin Antibody (SA30-04) [NBP2-67307]

Immunohistochemistry-Paraffin: beta-Catenin Antibody (SA30-04) [NBP2-67307] - Analysis of human colon cancer tissue using anti- Beta-Catenin antibody. Counter stained with hematoxylin.

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Applications for beta-Catenin Antibody (SA30-04)

Application

Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry-Paraffin

1:20-1:50

Multiplex Immunofluorescence

1:500-1:5000

Western Blot

1:500-1:5000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

TBS (pH7.4), 0.05% BSA, 40% Glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: beta-Catenin

The protein encoded by the beta Catenin gene is part of a complex of proteins that constitute adherens junctions (AJs). AJs arenecessary for the creation and maintenance of epithelial cell layers by regulating cell growth and adhesion betweencells. The encoded protein also anchors the actin cytoskeleton and may be responsible for transmitting the contactinhibition signal that causes cells to stop dividing once the epithelial sheet is complete. Finally, this proteinbinds to the product of the APC gene, which is mutated in adenomatous polyposis of the colon. Mutations in this geneare a cause of colorectal cancer (CRC), pilomatrixoma (PTR), medulloblastoma (MDB), and ovarian cancer. Three transcript variants encoding the same protein have been found for this gene.

Alternate Names

bCatenin, CTNNB1

Gene Symbol

CTNNB1

Additional beta-Catenin Products

Product Documents for beta-Catenin Antibody (SA30-04)

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Product Specific Notices for beta-Catenin Antibody (SA30-04)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.