Biotin-PEG4-Diazirine

Tocris Bioscience | Catalog # 8994

Photo-crosslinking capture probe for deazaflavin-diazirine energy-transfer labelling (DarT labelling) with a biotin handle
Tocris Bioscience
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Key Product Details

Description

Photo-crosslinking capture probe for deazaflavin-diazirine energy-transfer labelling (DarT labelling) with a biotin handle
View all Biochemicals and Molecular Biology Compounds »

Product Description

Key information:Biotin-PEG4-Diazirine is a cell-permeable photo-crosslinking probe for elucidating protein interactome of polyarginine cell-penetrating peptides and mapping cell surfaceome through deazaflavin-diazirine energy-transfer labelling (DarT labelling). Upon UV irradiation and in the presence of deazaflavin as an organic photocatalyst, the diazirine group is activated to a reactive carbene that covalently crosslinks to nearby proteins. The appended biotin handle allows for efficient affinity-based detection, pull-down, and isolation of tagged proteins from complex mixtures such as cell lysates. Suitable for live-cell applications.

Used for: photocatalytic proximity labelling.

Application: deazaflavin-diazirine energy-transfer labelling (DarT labelling) followed by affinity-based detection, pull-down, and isolation of tagged proteins from complex mixtures.

Product Specifications for Biotin-PEG4-Diazirine

Molecular Weight

688.75

Formula

C30H43F3N6O7S

Storage

Store at -20°C

Purity

≥95% (HPLC)

Chemical Name

1-(5-((3aS,4S,6aR)-2-Oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanamido)-N-(4-(3-(trifluoromethyl)-3H-diazirin-3-yl)benzyl)-3,6,9,12-tetraoxapentadecan-15-amide

InChI Key

MGUZAQIDWYWQHB-DPZBCOQUSA-N

SMILES

O=C1N[C@@]([H])([C@]([H])(CS2)N1)[C@@H]2CCCCC(NCCOCCOCCOCCOCCC(NCC3=CC=C(C4(N=N4)C(F)(F)F)C=C3)=O)=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Solubility

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 34.44 50 with gentle warming

Preparing Stock Solutions for Biotin-PEG4-Diazirine

The following data is based on the product molecular weight 688.75.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
0.5 mM 2.90 mL 14.52 mL 29.04 mL
2.5 mM 0.58 mL 2.90 mL 5.81 mL
5 mM 0.29 mL 1.45 mL 2.90 mL
25 mM 0.06 mL 0.29 mL 0.58 mL

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Background References

References are publications that support the biological activity of the product.

Product Documents for Biotin-PEG4-Diazirine

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Biotin-PEG4-Diazirine

For research use only

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