BubR1 Antibody (2G5) - Azide and BSA Free

Novus Biologicals | Catalog # H00000701-M02

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Sandwich ELISA, Immunocytochemistry/ Immunofluorescence, Proximity Ligation Assay, Knockdown Validated

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 2G5

Format

Azide and BSA Free
View all BubR1 Primary Antibodies »

Product Specifications

Immunogen

BUB1B (AAH18739, 1 a.a. ~ 130 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MAAVKKEGGALSEAMSLEGDEWELSKENVQPLRQGRIMSTLQGALAQESACNNTLQQQKRAFEYEIRFYTGNDPLDVWDRYISWTEQNYPQGGKESNMSTLLERAVEALQGEKRYYSDPRFLNLWLKLGR

Specificity

BUB1B - BUB1 budding uninhibited by benzimidazoles 1 homolog beta (yeast)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for BubR1 Antibody (2G5) - Azide and BSA Free

Western Blot: BubR1 Antibody (2G5) [H00000701-M02]

Western Blot: BubR1 Antibody (2G5) [H00000701-M02]

Western Blot: BubR1 Antibody (2G5) [H00000701-M02] - Analysis of BUB1B expression in transfected 293T cell line by BUB1B monoclonal antibody (M02), clone 2G5.Lane 1: BUB1B transfected lysate(119.5 KDa).Lane 2: Non-transfected lysate.
Immunohistochemistry-Paraffin: BubR1 Antibody (2G5) [H00000701-M02]

Immunohistochemistry-Paraffin: BubR1 Antibody (2G5) [H00000701-M02]

Immunohistochemistry-Paraffin: BubR1 Antibody (2G5) [H00000701-M02] - Analysis of monoclonal antibody to BUB1B on formalin-fixed paraffin-embedded human spleen. Antibody concentration 3 ug/ml.
Western Blot: BubR1 Antibody (2G5) [H00000701-M02]

Western Blot: BubR1 Antibody (2G5) [H00000701-M02]

Western Blot: BubR1 Antibody (2G5) [H00000701-M02] - Analysis of BUB1B over-expressed 293 cell line, cotransfected with BUB1B Validated Chimera RNAi ( Cat # H00000701-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with BUB1B monoclonal antibody (M02) clone 2G5 (Cat # H00000701-M02 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
Western Blot: BubR1 Antibody (2G5) [H00000701-M02]

Western Blot: BubR1 Antibody (2G5) [H00000701-M02]

Western Blot: BubR1 Antibody (2G5) [H00000701-M02] - BUB1B monoclonal antibody (M02), clone 2G5 Analysis of BUB1B expression in Hela S3 NE.
Proximity Ligation Assay: BubR1 Antibody (2G5) [H00000701-M02]

Proximity Ligation Assay: BubR1 Antibody (2G5) [H00000701-M02]

Proximity Ligation Assay: BubR1 Antibody (2G5) [H00000701-M02] - Analysis of protein-protein interactions between CDC20 and BUB1B. HeLa cells were stained with anti-CDC20 rabbit purified polyclonal 1:1200 and anti-BUB1B mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).
Sandwich ELISA: BubR1 Antibody (2G5) [H00000701-M02] - Detection limit for recombinant GST tagged BUB1B is approximately 0.03ng/ml as a capture antibody.

Applications for BubR1 Antibody (2G5) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IHC-P, RNAi Validation and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: BubR1

This gene encodes a kinase involved in spindle checkpoint function. The protein has been localized to the kinetochore and plays a role in the inhibition of the anaphase-promoting complex/cyclosome (APC/C), delaying the onset of anaphase and ensuring proper chromosome segregation. Impaired spindle checkpoint function has been found in many forms of cancer.

Alternate Names

Bub1A, BUBR1budding uninhibited by benzimidazoles 1 (yeast homolog), beta, budding uninhibited by benzimidazoles 1 homolog beta (yeast), EC 2.7.11.1, hBUBR1, MAD3/BUB1-related protein kinase, MAD3LBUB1beta, Mitotic checkpoint kinase MAD3L, Protein SSK1, SSK1mitotic checkpoint serine/threonine-protein kinase BUB1 beta

Entrez Gene IDs

701 (Human)

Gene Symbol

BUB1B

OMIM

114500 (Human)

UniProt

AAH18739 (Human)

Additional BubR1 Products

Product Documents for BubR1 Antibody (2G5) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for BubR1 Antibody (2G5) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for BubR1 Antibody (2G5) - Azide and BSA Free

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Protocols

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FAQs

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