Q: I am looking for some c-fos antibodies that could work in IHC.

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c-Fos Antibody (2H8M9)

Novus Biologicals | Catalog # NBP3-57387

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Product Documents

Recombinant Monoclonal Antibody

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry-Paraffin, Western Blot, ELISA

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 2H8M9

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Product Specifications

Immunogen

Recombinant protein (or fragment).This information is considered to be commercially sensitive. (Swissprot# P01100)

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

This antibody is highly specific and optimized for use in Immunohistochemistry.

Normal Tissues Tested
Fallopian tube, Pancreas, Lung, Kidney, Placenta, Stomach, Liver, Thyroid, Appendix, Testis, Prostate, Tonsil, Cervix, esophagus, Breast, Colon, Brain

Cancer Tissues Tested
Lung adenocarcinoma, Lung squamous carcinoma, Hepatocellular Carcinoma, Hepatocholangio carcinoma, Thyroid Cancer, Prostate cancer, cervical squamous cell carcinoma, Breast Cancer, Colon Cancer

Positive Controls
Low tissue specificity.

Negative Controls
Fallopian tube

Scientific Data Images for c-Fos Antibody (2H8M9)

Western Blot: c-Fos Antibody (2H8M9) [NBP3-57387]

Western blot analysis of lysates from NIH/3T3 cells using NBP3-57387 at 1:1000 dilution incubated overnight at 4C. NIH/3T3 cells were treated with PMA(200 nM) at 37C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 30 ug per lane. Blocking buffer: 3 % nonfat dry milk in TBST. Exposure time: 45s.

Western Blot: c-Fos Antibody (2H8M9) [NBP3-57387]

Western blot analysis of lysates from PC-12 cells using NBP3-57387 at 1:1000 dilution incubated overnight at 4C. PC-12 cells were treated with PMA(200 nM) at 37C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 30 ug per lane. Blocking buffer: 3 % nonfat dry milk in TBST. Exposure time: 60s.

Immunohistochemistry-Paraffin: c-Fos Antibody (2H8M9) [NBP3-57387]

Confocal imaging of paraffin-embedded Mouse brain tissue using NBP3-57387 (dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.

Immunohistochemistry-Paraffin: c-Fos Antibody (2H8M9) [NBP3-57387]

Confocal imaging of paraffin-embedded Mouse colon tissue using NBP3-57387 (dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.

Immunohistochemistry-Paraffin: c-Fos Antibody (2H8M9) [NBP3-57387]

Confocal imaging of paraffin-embedded Rat brain tissue using NBP3-57387 (dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.

Western Blot: c-Fos Antibody (2H8M9) [NBP3-57387]

Western blot analysis of lysates from HeLa cells using NBP3-57387 at 1:1000 dilution incubated overnight at 4C. HeLa cells were treated with PMA(200 nM) at 37C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3 % nonfat dry milk in TBST. Exposure time: 45s.

Immunocytochemistry/ Immunofluorescence: c-Fos Antibody (2H8M9) [NBP3-57387]

Confocal imaging of HeLa cells (treated with PMA) and HeLa cells (untreated) using NBP3-57387 (dilution 1:200) followed by a further incubation with Cy3-conjugated Goat anti-Rabbit IgG (H+L) (dilution 1:500) (Red). The cells were counterstained with alpha-Tubulin Mouse mAb (dilution 1:400) followed by incubation with conjugated Goat Anti-Mouse IgG (H+L) (dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.

Immunohistochemistry-Frozen: c-Fos Antibody (2H8M9) [NBP3-57387]

Confocal imaging of frozen sections of Mouse brain tissue using NBP3-57387 (dilution 1:2000) followed by a further incubation with Cy3-conjugated Goat anti-Rabbit IgG (H+L) (dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Microwave antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.

Immunohistochemistry-Frozen: c-Fos Antibody (2H8M9) [NBP3-57387]

Confocal imaging of frozen sections of Rat brain tissue using NBP3-57387 (dilution 1:2000) followed by a further incubation with Cy3-conjugated Goat anti-Rabbit IgG (H+L) (dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Microwave antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.

Immunohistochemistry-Paraffin: c-Fos Antibody (2H8M9) [NBP3-57387]

Immunohistochemistry analysis of paraffin-embedded Human cervix using NBP3-57387 at a dilution of 1:400 (40x lens). High pressure antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.

Immunohistochemistry-Paraffin: c-Fos Antibody (2H8M9) [NBP3-57387]

Immunohistochemistry analysis of paraffin-embedded Human colon using NBP3-57387 at a dilution of 1:400 (40x lens). High pressure antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.

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Applications for c-Fos Antibody (2H8M9)

Application

Recommended Usage

ELISA

1 ug/mL

Immunohistochemistry-Paraffin

1:200 - 1:800

Western Blot

1:1000 - 1:4000

Application Notes

For immunofluorescence on paraffin-embedded tissues, the recommended concentration is 1:200 - 1:400

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 50% glycerol, 0.05% BSA, (pH 7.3)

Preservative

0.09% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: c-Fos

c-Fos is an intermediate early gene that is one of four members of the FOS family of activator protein-1 (AP-1) transcription factors, which also includes Fra-1, Fra-2, and FosB (1-3). Under the FOS gene, human c-Fos is synthesized as a protein of 308 amino acids (aa) with a basic leucine zipper (bZip) domain important for dimerization, and a basic domain for interacting with DNA, with a theoretical molecular weight of ~40.6 kDa (4,5). c-Fos can heterodimerize with members of the JUN family (c-Jun, JunB, and JunD) to form the active transcription factor AP-1 complex (3,5). AP-1 related proteins bind TPA-related responsive elements (TRE), cAMP responsive elements (CRE), and related sequences, with c-Fos:c-Jun dimers partial to TRE sites (5).

In response to stimuli, c-Fos, which is encoded by protooncogenes, has a role in cell proliferation, differentiation, and transformation (3,6). A variety of stimuli can increase c-Fos expression such as growth factors, proinflammatory cytokines, UV radiation, neurotransmitters, hormones, injury, and stress (1,6). c-Fos has long been used as a marker for neuronal activity and is associated with neural and behavioral responses following stimuli (1-3, 6-7). Mouse studies have revealed that c-Fos is important for efficient neurogenesis and cortical development (3). Additionally, c-Fos signal can be used as a molecular marker for learning and memory, such as recognition and fear (2,7). Studies have found that repeated positive stimuli result in increased Fos expression while, conversely, repeated negative value stimuli are indicated by decreased signal (7). Intermediate early genes have also been implicated in neuropsychiatric disorders including showing altered c-Fos expression in a schizophrenia animal model (2). Furthermore, antipsychotics and antidepressants are both capable of impacting c-Fos expression (2).

References

1. Kovacs K. J. (1998). c-Fos as a transcription factor: a stressful (re)view from a functional map. Neurochemistry International. https://doi.org/10.1016/s0197-0186(98)00023-0

2. Gallo, F. T., Katche, C., Morici, J. F., Medina, J. H., & Weisstaub, N. V. (2018). Immediate Early Genes, Memory and Psychiatric Disorders: Focus on c-Fos, Egr1 and Arc. Frontiers in Behavioral Neuroscience. https://doi.org/10.3389/fnbeh.2018.00079

3. Velazquez, F. N., Caputto, B. L., & Boussin, F. D. (2015). c-Fos importance for brain development. Aging. https://doi.org/10.18632/aging.100862

4. Uniprot (P01100)

5. Wu, Z., Nicoll, M., & Ingham, R. J. (2021). AP-1 family transcription factors: a diverse family of proteins that regulate varied cellular activities in classical hodgkin lymphoma and ALK+ ALCL. Experimental Hematology & Oncology. https://doi.org/10.1186/s40164-020-00197-9

6. Shaulian, E., & Karin, M. (2001). AP-1 in cell proliferation and survival. Oncogene. https://doi.org/10.1038/sj.onc.1204383

7. Chung L. (2015). A Brief Introduction to the Transduction of Neural Activity into Fos Signal. Development & Reproduction. https://doi.org/10.12717/DR.2015.19.2.061

Long Name

FBJ/Finkel–Biskis–Jinkins Murine Osteosarcoma Viral Oncogene Homolog

Alternate Names

cFos, FOS, G0S7

Gene Symbol

FOS

Additional c-Fos Products

Product Documents for c-Fos Antibody (2H8M9)

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Certificate of Analysis

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Product Specific Notices for c-Fos Antibody (2H8M9)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Apoptosis Transcription Factors and Regulators

Basic Leucine Zipper (bZIP) Transcription Factors

C-type Lectin Receptors

IL-1 Family

NOD-like Receptors and the Inflammasome

PDGF Family

Toll-Like Receptors

Transcription Factors in the Akt Pathway

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Protocols

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FAQs for c-Fos Antibody (2H8M9)

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Q: I am looking for some c-fos antibodies that could work in IHC.

A:
Here are a list of products which might be of interest to you: Product List. We have additional filters on the left which may help you narrow down based on host species, target species, clonality, etc. We will guarantee these to work for all stated species and applications listed on the datasheet.