Recombinant Bovine C-Reactive Protein/CRP Protein

Novus Biologicals | Catalog # NBP3-57610

Novus Biologicals
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Key Product Details

Applications

Western Blot, ELISA, Cell Culture
View all C-Reactive Protein/CRP Proteins and Enzymes »

Product Specifications

Description

A recombinant protein corresponding to 205 aa of Bovine C-Reactive Protein/CRP.

Source: Yeast

Amino Acid Sequence: QTDLHKKAFV FPKETENSYV SLKTQLRTPL KAFTVCLYFY TELARTHSYS IFSYATKQQP NEILIFWSKG KGYIFGVHGK QVLFQSPENT QAPTHICASW ESTSGIAELW VNGKPRMRKS LEKGAILGTE ASIILGQEQD AFAGGFDRNQ CLVGDIGDVN MWDFVLSPEE INAVYLGSTL SPNVLNWQAL NYEAKGEVFI KPQLW (205)

Stable for up to 12 months from date of receipt at -20C. Stable for 3 months when stored in working aliquots with a carrier protein at -20C. Avoid repeated freeze/thaw cycles.

Purity

>98%, by SDS-PAGE

Endotoxin Level

No affinity tags & naturally endotoxin-free

Predicted Molecular Mass

23.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Applications

Enzyme-linked Immunosorbent Spot (ELISPOT)
ELISA Standard (Matched Pair) (ELISA(Sta))

Protein / Peptide Type

Recombinant Protein

Formulation, Preparation, and Storage

NBP3-57610
Formulation Lyophilized in 1-5X PBS containing 10% Trehalose
Preservative 10% Trehalose
Concentration Lyoph
Reconstitution Reconstitute in sterile PBS with at least 0.1% carrier protein
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at -20C. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: C-Reactive Protein/CRP

C Reactive Protein is a major acute phase reactant synthesized primarily in the liver hepatocytes. It is a pentraxin (cyclic pentameric protein) compound of five identical nonglycosylated subunits of 206 amino acids each (m.w. 24 kDa), that are bound noncovalently to form the physiologic CRP molecule (m.w. 117.5 kDa). C Reactive Protein mediates activities associated with preimmune nonspecific host resistance. It is opsonic, an initiator of the classical complement cascade and an activator of monocytes/macrophages. CRP also binds to several nuclear components including chromatin, histones and snRNP, suggesting that it may play a role as a scavenger during cell necrosis. Studies have revealed that among other markers of inflammation, CRP shows the strongest association with cardiovascular events. Many clinical studies demonstrated that coronary mortality among patients with unstable angina and elevated CRP is significantly higher comparing with the patients without elevated CRP. Measurements of C reactive protein (hsCRP) in the patients with ischemic heart disease provide a novel method for detecting individuals at high risk of plaque rupture.

Alternate Names

CRP

Gene Symbol

CRP

Additional C-Reactive Protein/CRP Products

Product Documents for Recombinant Bovine C-Reactive Protein/CRP Protein

Certificate of Analysis

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Product Specific Notices for Recombinant Bovine C-Reactive Protein/CRP Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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Protocols

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