Cadherin-17 Antibody (5F5) - Chimeric

Novus Biologicals | Catalog # NBP3-48406

Recombinant Monoclonal Antibody
Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, Flow Cytometry

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Human IgG1 Clone # 5F5
View all Cadherin-17 Primary Antibodies »

Product Specifications

Immunogen

Cadherin-17 recombinant protein expressed in mammalian cells (Accession# Q12864). The exact amino acid sequence is proprietary.

Clonality

Monoclonal

Host

Human

Isotype

IgG1

Description

This product is shipped at ambient temperature. Store at -20C to -80C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80C (Avoid repeated freezing and thawing).

Scientific Data Images for Cadherin-17 Antibody (5F5) - Chimeric

Cadherin-17 Antibody (5F5) - Chimeric

Western Blot: Cadherin-17 Antibody (5F5) - Chimeric [NBP3-48406]

Anti-CDH17 antibody (Catalog Number# NBP3-48406) at 1/1000 dilutionLane 1: AGS, whole cell lysateLane 2: HT55, whole cell lysateSecondary : Goat Anti-Rabbit IgG H&L (HRP) at 1/5000 dilutionPredicted band size: 92 kDaObserved band size: 120 kDa
Cadherin-17 Antibody (5F5) - Chimeric

Flow Cytometry: Cadherin-17 Antibody (5F5) - Chimeric [NBP3-48406]

Flow cytometry analysis of antigen binding of anti-human CDH17 mAb(NBP3-48406). (A) NBP3-48406 does not bind to 293T cells that do not express CDH17 (B) A clear peak shift of NBP3-48406 was seen compared to the control when incubated with CDH17 -expressing HT55 cells, indicating strong binding of NBP3-48406 to CDH17. Antibodies were incubated at 2ug/mL.

Applications for Cadherin-17 Antibody (5F5) - Chimeric

Application
Recommended Usage

Flow Cytometry

1:100

Western Blot

1:1000

Flow Cytometry Panel Builder

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Advanced Features

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  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified from cell culture supernatant

Reconstitution

Dissolve the lyophilized antibody in sterile and low endotoxin water. The recommend volume of water is lot specific. Please refer to the instructions printed on the product vial or contact technical support.

Formulation

PBS, pH 7.4, 5-8% Trehalose

Preservative

Trehalose

Concentration

LYOPH mg/ml

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C in powder form. Store at -80C once reconstituted.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Cadherin-17

LI Cadherin is a member of the cadherin superfamily, genes encoding calcium-dependent, membrane-associated glycoproteins. The encoded protein is cadherin-like, consisting of an extracellular region, containing 7 cadherin domains, and a transmembrane region but lacking the conserved cytoplasmic domain. The protein is a component of the gastrointestinal tract and pancreatic ducts, acting as an intestinal proton-dependent peptide transporter in the first step in oral absorption of many medically important peptide-based drugs. The protein may also play a role in the morphological organization of liver and intestine.

Alternate Names

Cadherin17, CDH17

Gene Symbol

CDH17

Additional Cadherin-17 Products

Product Documents for Cadherin-17 Antibody (5F5) - Chimeric

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Cadherin-17 Antibody (5F5) - Chimeric

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Adhesion Molecules
Cadherin Superfamily

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