Calnexin Antibody (IE2.1C12) - Azide and BSA Free
Novus Biologicals | Catalog # NBP2-80609
![Western Blot: Calnexin Antibody (IE2.1C12)Azide and BSA Free [NBP2-80609]](https://resources.rndsystems.com/images/products/Calnexin-Antibody-IE2-1C12-Azide-and-BSA-Free-Western-Blot-NBP2-80609-img0001.jpg "Western Blot: Calnexin Antibody (IE2.1C12)Azide and BSA Free [NBP2-80609]")
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Flow (Intracellular), Immunocytochemistry/ Immunofluorescence, CyTOF-ready
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2b Kappa Clone # IE2.1C12
Format
Azide and BSA Free
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Product Specifications
Immunogen
Partial recombinant human Calnexin protein (between amino acids 1-300) [UniProt# P27824]
Localization
Endoplasmic reticulum membrane, Melanosome
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2b Kappa
Scientific Data Images for Calnexin Antibody (IE2.1C12) - Azide and BSA Free
Western Blot: Calnexin Antibody (IE2.1C12)Azide and BSA Free [NBP2-80609]
Western Blot: Calnexin Antibody (IE2.1C12) - Azide and BSA Free [NBP2-80609] - Analysis of 11 kDa Partial Recombinant Human Calnexin protein with Calnexin antibody (clone IE2.1C12) at 0.5 ug/ml concentration. Image from the standard format of this antibody.![Immunocytochemistry: Calnexin Antibody (IE2.1C12) - Azide and BSA Free [NBP2-80609]](https://resources.rndsystems.com/images/products/Calnexin-Antibody-IE2-1C12-Azide-and-BSA-Free-Immunocytochemistry-NBP2-80609-img0003.jpg "Immunocytochemistry: Calnexin Antibody (IE2.1C12) - Azide and BSA Free [NBP2-80609]")
Immunocytochemistry: Calnexin Antibody (IE2.1C12) - Azide and BSA Free [NBP2-80609]
Immunocytochemistry: Calnexin Antibody (IE2.1C12) - Azide and BSA Free [NBP2-80609] - Analysis of FFPE tissue section of human placenta using mouse monoclonal Calnexin antibody (clone IE2.1C12) at 7 ug/ml concentration. Image from the standard format of this antibody.![Immunohistochemistry-Paraffin: Calnexin Antibody (IE2.1C12) - Azide and BSA Free [NBP2-80609]](https://resources.rndsystems.com/images/products/Calnexin-Antibody-IE2-1C12-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-80609-img0007.jpg "Immunohistochemistry-Paraffin: Calnexin Antibody (IE2.1C12) - Azide and BSA Free [NBP2-80609]")
Immunohistochemistry-Paraffin: Calnexin Antibody (IE2.1C12) - Azide and BSA Free [NBP2-80609]
Immunohistochemistry-Paraffin: Calnexin Antibody (IE2.1C12) - Azide and BSA Free [NBP2-80609] - Analysis of FFPE tissue section of normal human kidney using mouse monoclonal Calnexin antibody (clone IE2.1C12) at 7 ug/ml concentration. The cells of Glomeruli developed strong cytoplasmic staining. Image from the standard format of this antibody.![Flow Cytometry: Calnexin Antibody (IE2.1C12) - Azide and BSA Free [NBP2-80609]](https://resources.rndsystems.com/images/products/Calnexin-Antibody-IE2-1C12-Azide-and-BSA-Free-Flow-Cytometry-NBP2-80609-img0008.jpg "Flow Cytometry: Calnexin Antibody (IE2.1C12) - Azide and BSA Free [NBP2-80609]")
Flow Cytometry: Calnexin Antibody (IE2.1C12) - Azide and BSA Free [NBP2-80609]
Flow Cytometry: Calnexin Antibody (IE2.1C12) - Azide and BSA Free [NBP2-80609] - Analysis of Allophycocyanin conjugate of NBP2-36571. An intracellular stain was performed on Jurkat cells with Calnexin antibody (IE2.1C12) NBP2-36571APC (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin.![Immunocytochemistry/ Immunofluorescence: Calnexin Antibody (IE2.1C12) - Azide and BSA Free [NBP2-80609]](https://resources.rndsystems.com/images/products/Calnexin-Antibody-IE2-1C12-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-80609-img0002.jpg "Immunocytochemistry/ Immunofluorescence: Calnexin Antibody (IE2.1C12) - Azide and BSA Free [NBP2-80609]")
Immunocytochemistry/ Immunofluorescence: Calnexin Antibody (IE2.1C12) - Azide and BSA Free [NBP2-80609]
Immunocytochemistry/Immunofluorescence: Calnexin Antibody (IE2.1C12) - Azide and BSA Free [NBP2-80609] - Calnexin (1E2.1C12) antibody was tested in Hela cells with DyLight488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red). Image from the standard format of this antibody.![Immunohistochemistry-Paraffin: Calnexin Antibody (IE2.1C12) - Azide and BSA Free [NBP2-80609]](https://resources.rndsystems.com/images/products/Calnexin-Antibody-IE2-1C12-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-80609-img0004.jpg "Immunohistochemistry-Paraffin: Calnexin Antibody (IE2.1C12) - Azide and BSA Free [NBP2-80609]")
Immunohistochemistry-Paraffin: Calnexin Antibody (IE2.1C12) - Azide and BSA Free [NBP2-80609]
Immunohistochemistry-Paraffin: Calnexin Antibody (IE2.1C12) - Azide and BSA Free [NBP2-80609] - Analysis of FFPE tissue section of human skin using mouse monoclonal Calnexin antibody (clone IE2.1C12) at 7 ug/ml concentration. The outermost keratinocytes layer of the epidermis showed cytoplasmic positivity for Calnexin protein. Image from the standar![Immunohistochemistry-Paraffin: Calnexin Antibody (IE2.1C12) - Azide and BSA Free [NBP2-80609]](https://resources.rndsystems.com/images/products/Calnexin-Antibody-IE2-1C12-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-80609-img0005.jpg "Immunohistochemistry-Paraffin: Calnexin Antibody (IE2.1C12) - Azide and BSA Free [NBP2-80609]")
Immunohistochemistry-Paraffin: Calnexin Antibody (IE2.1C12) - Azide and BSA Free [NBP2-80609]
Immunohistochemistry-Paraffin: Calnexin Antibody (IE2.1C12) - Azide and BSA Free [NBP2-80609] - Analysis of FFPE tissue section of malignant stromal tumor of the human small bowel using mouse monoclonal Calnexin antibody (clone IE2.1C12) at 7 ug/ml concentration. The cancer cells showed a very strong cytoplasmic reactivity for Calnexin. Image from t![Immunohistochemistry-Paraffin: Calnexin Antibody (IE2.1C12) - Azide and BSA Free [NBP2-80609]](https://resources.rndsystems.com/images/products/Calnexin-Antibody-IE2-1C12-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-80609-img0006.jpg "Immunohistochemistry-Paraffin: Calnexin Antibody (IE2.1C12) - Azide and BSA Free [NBP2-80609]")
Immunohistochemistry-Paraffin: Calnexin Antibody (IE2.1C12) - Azide and BSA Free [NBP2-80609]
Immunohistochemistry-Paraffin: Calnexin Antibody (IE2.1C12) - Azide and BSA Free [NBP2-80609] - Analysis of FFPE tissue section of normal human breast using mouse monoclonal Calnexin antibody (clone IE2.1C12) at 7 ug/ml concentration. The myoepithelial cells around the lobules depicted a very strong cytoplasmic staining. Image from the standard formApplications for Calnexin Antibody (IE2.1C12) - Azide and BSA Free
Application
Recommended Usage
Flow (Intracellular)
5 ug/million cells
Immunocytochemistry/ Immunofluorescence
10 ug/ml
Immunohistochemistry
7 ug/ml
Flow Cytometry Panel Builder
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Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Calnexin
Alternate Names
calnexin, CNX, IP90FLJ26570, Major histocompatibility complex class I antigen-binding protein p88, P90
Gene Symbol
CANX
Additional Calnexin Products
Product Documents for Calnexin Antibody (IE2.1C12) - Azide and BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Calnexin Antibody (IE2.1C12) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- 7-Amino Actinomycin D (7-AAD) Cell Viability Flow Cytometry Protocol
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Extracellular Membrane Flow Cytometry Protocol
- Flow Cytometry Protocol for Cell Surface Markers
- Flow Cytometry Protocol for Staining Membrane Associated Proteins
- Flow Cytometry Staining Protocols
- Flow Cytometry Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Intracellular Flow Cytometry Protocol Using Alcohol (Methanol)
- Intracellular Flow Cytometry Protocol Using Detergents
- Intracellular Nuclear Staining Flow Cytometry Protocol Using Detergents
- Intracellular Staining Flow Cytometry Protocol Using Alcohol Permeabilization
- Intracellular Staining Flow Cytometry Protocol Using Detergents to Permeabilize Cells
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Propidium Iodide Cell Viability Flow Cytometry Protocol
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Liperfluo
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Characterization of Human Th22 Cells
- Protocol for the Characterization of Human Th9 Cells
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- Protocol: Annexin V and PI Staining by Flow Cytometry
- Protocol: Annexin V and PI Staining for Apoptosis by Flow Cytometry
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Fluorokine Flow Cytometry Kits
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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