CaM Kinase II alpha Antibody

Novus Biologicals | Catalog # NBP1-51945

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Peptide ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Cited:

Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all CaM Kinase II alpha Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-PRTAQSEETRVWHR, from the internal region of the protein sequence according to NP_057065.2; NP_001280099.1.

Epitope

C-PRTAQSEETRVWHR

Specificity

This antibody is expected to recognize both reported isoforms (NP_057065.2 (CAMK2A) and NP_001280099.1 (CAMK2B).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for CaM Kinase II alpha Antibody

Immunohistochemistry: CaM Kinase II alpha Antibody [NBP1-51945]

Immunohistochemistry: CaM Kinase II alpha Antibody [NBP1-51945]

Immunohistochemistry: CaM Kinase II alpha Antibody [NBP1-51945] - IHC using CamKII antibody from goat in 3% PFA perfused mouse brain. 1:100 dilution clearly provides cytosolic CamK II immunoreactivity in whole brain regions, including the hippocampus (HP), cortex (CTX) and paraventricular region (PVN). Image from verified customer review.
CaM Kinase II alpha Antibody

Flow Cytometry: CaM Kinase II alpha Antibody [NBP1-51945] -

Flow Cytometry: CaM Kinase II alpha Antibody [NBP1-51945] - Flow cytometric analysis of paraformaldehyde fixed Kelly cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
CaM Kinase II alpha Antibody

Immunocytochemistry/Immunofluorescence: CaM Kinase II alpha Antibody [NBP1-51945] -

Immunocytochemistry/Immunofluorescence: CaM Kinase II alpha Antibody [NBP1-51945] - Immunofluorescence analysis of paraformaldehyde fixed U251 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
CaM Kinase II alpha Antibody

Immunohistochemistry-Paraffin: CaM Kinase II alpha Antibody [NBP1-51945] -

Immunohistochemistry-Paraffin: CaM Kinase II alpha Antibody [NBP1-51945] - (3.75ug/ml) staining of paraffin embedded Human Adrenal Gland. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
CaM Kinase II alpha Antibody

Immunohistochemistry-Paraffin: CaM Kinase II alpha Antibody [NBP1-51945] -

Immunohistochemistry-Paraffin: CaM Kinase II alpha Antibody [NBP1-51945] - (3.8ug/ml) staining of paraffin embedded Human Brain Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
CaM Kinase II alpha Antibody

Western Blot: CaM Kinase II alpha Antibody [NBP1-51945] -

Western Blot: CaM Kinase II alpha Antibody [NBP1-51945] - (0.3ug/ml) staining of Human Cerebellum (A), Mouse (B) and Rat (C) Brain lysate (35ug protein in RIPA buffer). Detected by chemiluminescence
CaM Kinase II alpha Antibody

Immunocytochemistry/Immunofluorescence: CaM Kinase II alpha Antibody [NBP1-51945] -

Immunocytochemistry/Immunofluorescence: CaM Kinase II alpha Antibody [NBP1-51945] - Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

Applications for CaM Kinase II alpha Antibody

Application
Recommended Usage

Flow Cytometry

10 ug/ml

Immunocytochemistry/ Immunofluorescence

10 ug/ml

Immunohistochemistry

3 - 6 ug/ml

Immunohistochemistry-Paraffin

3 - 6 ug/ml

Peptide ELISA

Detection limit 1:64000

Western Blot

0.3-1 ug/ml
Application Notes
WB: Approx. 60kDa band observed in Human Cerebellum and approx 50+60kDa in Mouse and Rat Brain lysates, corresponding to CAMK2alpha and CAMK2beta respectively (calculated MW of 55.3kDa according to Human NP_057065.2 and 54.1kDa according to Mouse NP_803126.1 and Rat NP_037052.1 subunit Alpha and 60.4kDa according to Human NP_001280099.1, Mouse : NP_031621.3 and Rat NP_068507.2 subunit beta).. These observed molecular weights are routinely observed by other sources.and were successfully blocked by incubation with the immunizing peptide. Primary incubation 1 hour at room temperature. IHC-P: Human brain cortex shows granular staining in the periphery of pyramydal cell bodies.

Reviewed Applications

Read 1 review rated 4 using NBP1-51945 in the following applications:

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: CaM Kinase II alpha

Calcium/calmodulin-dependent protein kinase II (CaMKII) is a widely distributed protein kinase that regulates numerous physiological functions. Inhibitors of CaMKII are useful tools for investigating the CaMKII functions (1). The synapse contains densely localized and interacting proteins that enable it to adapt to changing inputs. A Ca2+-sensitive protein complex involved in the regulation of AMPA receptor synaptic plasticity. The complex is comprised of MUPPI, a multi-PDZ domain-containing protein; SynGAP, a synaptic GTPase-activating protein; and the Ca2+/calmodulin-dependent kinase CaMKII. In synapses of hippocampal neurons, SynGAP and CaMKII are brought together by direct physical interaction with the PDZ domains of MUPP1, and in this complex, SynGAP is phosphorylated. Ca2+CaM binding to CaMKII dissociates it from the MUPP1 complex, and Ca2+ entering via the NMDAR drives the dephosphorylation of SynGAP (2).

Long Name

Calcium/Calmodulin-dependent Protein Kinase II alpha

Alternate Names

CAMK2A

Entrez Gene IDs

815 (Human); 12322 (Mouse); 25400 (Rat)

Gene Symbol

CAMK2A

UniProt

NP_057065.2 (Human)

Additional CaM Kinase II alpha Products

Product Documents for CaM Kinase II alpha Antibody

Certificate of Analysis

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Product Specific Notices for CaM Kinase II alpha Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for CaM Kinase II alpha Antibody

Customer Reviews for CaM Kinase II alpha Antibody (1)

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  • CaM Kinase II alpha Antibody
    Name: Hayk Simonyan
    Application: Immunohistochemistry
    Sample Tested: Mouse brain
    Species: Mouse
    Verified Customer | Posted 04/30/2018
    IHC using CamKII antibody from goat in 3% PFA perfused mouse brain. 1:100 dilution clearly provides cytosolic CamK II immunoreactivity in whole brain regions, including the hippocampus (HP), cortex (CTX) and paraventricular region (PVN).
    CaM Kinase II alpha Antibody NBP1-51945

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