Carm1 Antibody - BSA Free

Western Blot: Carm1 Antibody [NB200-342]

Western Blot: Carm1 Antibody [NB200-342] - Whole cell lysate (10 ug) from HEK293T, Jurkat, and HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CARM1 antibody used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.

Immunohistochemistry-Paraffin: Carm1 Antibody [NB200-342]

Immunohistochemistry-Paraffin: Carm1 Antibody [NB200-342] - Section of human stomach carcinoma. Antibody: Affinity purified rabbit anti-used at a dilution of 1:1,000 (1ug/ml). Detection: DAB

Immunoprecipitation: Carm1 Antibody [NB200-342]

Immunoprecipitation: Carm1 Antibody [NB200-342] - Detection of human CARM1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-CARM1 antibody NB200-342 (lot NB200-342-4) used for IP at 6 ug per reaction. CARM1 was also immunoprecipitated by a previous lot of this antibody (lot NB200-342-3) and another rabbit anti-CARM1 antibody. For blotting immunoprecipitated CARM1, NB200-342 was used at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.

Western Blot: Carm1 Antibody - BSA Free [NB200-342] -

CARM1 is upregulated in HCC tissues and indicates poor clinical outcomes.A Western blotting analysis showed the expression of CARM1 in 35 pairs of HCC and adjacent normal liver tissues. B The expression of CARM1 in 66 pairs of HCC and corresponding adjacent normal liver tissues was examined via an IHC assay. C Kaplan-Meier survival analysis of overall survival in HCC patients stratified by CARM1 expression. Patients with low expression (n = 19) had lower expression values in HCC tissues than in normal tissues, while patients with high expression (n = 47) had higher expression values in HCC tissues than in normal tissues. D CARM1 mRNA levels in normal and primary HCC tumor tissues from the TCGA database. E Kaplan-Meier survival analysis of HCC patients from the TCGA database was performed according to CARM1 mRNA expression. Patients with high CARM1 expression had expression values in the >3rd quantile, while patients with low CARM1 expression had expression values in the <3rd quartile. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/40016178), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: Carm1 Antibody - BSA Free [NB200-342] -

CARM1 promotes the proliferation of HCC cells.A Western blotting analysis showed the knockdown efficacy of CARM1 in Huh7 and PLC/PRF/5 cells infected with lentiviral particles expressing shRNAs targeting CARM1. B Proliferation of control and CARM1-knockdown Huh7 cells was detected by CCK-8 assays on the indicated days. C Colony formation assays were performed to detect the proliferation of control and CARM1-knockdown HCC cells. The data are presented in a bar chart. D Control or CARM1-knockdown Huh7 cells were subcutaneously injected into nude mice for observation of tumor growth. E The tumor volume was measured every three days and is presented as a line graph. F The tumor weights of the xenografts from the different groups were calculated. G Immunohistochemical analysis of mouse subcutaneous tumors was performed with anti-CARM1 and anti-Ki-67 antibodies. (n = 3; *p < 0.05, **p < 0.01, and ***p < 0.001). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/40016178), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunohistochemistry: Carm1 Antibody - BSA Free [NB200-342] -

CARM1 promotes the proliferation of HCC cells.A Western blotting analysis showed the knockdown efficacy of CARM1 in Huh7 and PLC/PRF/5 cells infected with lentiviral particles expressing shRNAs targeting CARM1. B Proliferation of control and CARM1-knockdown Huh7 cells was detected by CCK-8 assays on the indicated days. C Colony formation assays were performed to detect the proliferation of control and CARM1-knockdown HCC cells. The data are presented in a bar chart. D Control or CARM1-knockdown Huh7 cells were subcutaneously injected into nude mice for observation of tumor growth. E The tumor volume was measured every three days and is presented as a line graph. F The tumor weights of the xenografts from the different groups were calculated. G Immunohistochemical analysis of mouse subcutaneous tumors was performed with anti-CARM1 and anti-Ki-67 antibodies. (n = 3; *p < 0.05, **p < 0.01, and ***p < 0.001). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/40016178), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunohistochemistry: Carm1 Antibody - BSA Free [NB200-342] -

CARM1 is a downstream effector of PSMD14 that affects the prognosis of HCC patients.A CARM1 was transfected into HCC cells with PSMD14 knockdown. A CCK-8 assay was performed to detect proliferation. B CARM1 was transfected into HCC cells with PSMD14 knockdown. Then, a colony formation assay was conducted. C CARM1 was transfected into HCC cells with PSMD14 knockdown. Then, a transwell assay was performed to detect migration and invasion. Representative images of the transwell assay are shown. The cells in five randomly selected fields were counted under a microscope. D Representative images of immunohistochemical staining of PSMD14 and CARM1 in the same HCC and corresponding adjacent normal liver tissues are shown. E Correlation analysis of PSMD14 and CARM1 in HCC tissues. The data were statistically analyzed by the Chi-square test. R indicates the Pearson correlation coefficient. F Scatter diagram showing a positive correlation between PSMD14 and CARM1 in HCC tissues by IHC. G Survival analysis of HCC patients was conducted using Kaplan-Meier plots and log-rank tests. The patients were categorized into high and low PSMD14 and CARM1 expression groups based on IHC staining. (n = 3; *p < 0.05, **p < 0.01, and ***p < 0.001). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/40016178), licensed under a CC-BY license. Not internally tested by Novus Biologicals.