Cathepsin B Antibody (23GB1130)

Novus Biologicals | Catalog # NBP3-52045

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence, Knockdown Validated

Label

Unconjugated

Antibody Source

Monoclonal Rabbit IgG Clone # 23GB1130
View all Cathepsin B Primary Antibodies »

Product Specifications

Immunogen

A synthesized peptide derived from human Cathepsin B (Uniprot: P07858)

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Cathepsin B Antibody (23GB1130)

Cathepsin B Antibody (23GB1130)

Immunocytochemistry/ Immunofluorescence: Cathepsin B Antibody (23GB1130)[NBP3-52045] -

Immunocytochemical staining of HepG2 cells with Cathepsin B antibody (1:1,000). Nuclei were stained blue with DAPI; Cathepsin B was stained magenta with Alexa Fluor(R) 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar, 20 um.
Cathepsin B Antibody (23GB1130)

Western Blot: Cathepsin B Antibody (23GB1130)[NBP3-52045] -

Western blotting analysis using Cathepsin B antibody (1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using FeQ(TM) ECL Substrate Kit.
Cathepsin B Antibody (23GB1130)

Western Blot: Cathepsin B Antibody (23GB1130)[NBP3-52045] -

Western blotting analysis using Cathepsin B antibody (1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using NaQ(TM) ECL Substrate Kit.

Applications for Cathepsin B Antibody (23GB1130)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Knockdown Validated

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.4), 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Cathepsin B

The cathepsin family of proteolytic enzymes contains several diverse classes of proteases. The cysteine protease class comprises cathepsins B, L, H, K, S and O. The aspartyl protease class is composed of cathepsins D and E. cathepsin G is in the serine protease class. Most cathepsins are lysosomal and each is involved in cellular metabolism, participating in various events such as peptide biosynthesis and protein degradation. cathepsin B is expressed in luminal epithelial cells, indicating that cathepsin B is a marker for secretory cell death.

Alternate Names

CTSB

Gene Symbol

CTSB

Additional Cathepsin B Products

Product Documents for Cathepsin B Antibody (23GB1130)

Certificate of Analysis

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Product Specific Notices for Cathepsin B Antibody (23GB1130)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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