![Immunohistochemistry-Paraffin: CBLB Antibody [NBP1-49919]](https://resources.rndsystems.com/images/products/CBLB-Antibody-Immunohistochemistry-NBP1-49919-img0004.jpg "Immunohistochemistry-Paraffin: CBLB Antibody [NBP1-49919]")
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Scientific Data Images for CBLB Antibody - BSA Free
Immunohistochemistry-Paraffin: CBLB Antibody [NBP1-49919]
Immunohistochemistry-Paraffin: CBLB Antibody [NBP1-49919] - Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti- Cbl-b used at a dilution of 1:1,000 (1ug/ml). Detection: DAB![Immunoprecipitation: CBLB Antibody [NBP1-49919]](https://resources.rndsystems.com/images/products/CBLB-Antibody-Immunoprecipitation-NBP1-49919-img0003.jpg "Immunoprecipitation: CBLB Antibody [NBP1-49919]")
Immunoprecipitation: CBLB Antibody [NBP1-49919]
Immunoprecipitation: CBLB Antibody [NBP1-49919] - Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-Cbl-b antibody NBP1-49919 used for IP at 3 ug/mg lysate. Cbl-b was also immunoprecipitated by rabbit anti-Cbl-b antibody NBP1-49920, which recognizes a downstream epitope. For blotting immunoprecipitated Cbl-b, NBP1-49920 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.Applications for CBLB Antibody - BSA Free
Immunohistochemistry
Immunohistochemistry-Paraffin
Immunoprecipitation
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Background: Cbl-B
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Additional Cbl-B Products
Product Documents for CBLB Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for CBLB Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Immunoprecipitation Protocol
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
FAQs for CBLB Antibody - BSA Free
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Q: One of our customers bought one vial of NBP1-49919, and he told me the website said it can be used in WB, but when he got the antibody, he find the website said as follows, the customer is a little confused about the WB (-). Does this mean that it can't be used in WB? Please give us some help and thanks.
A: Regarding your customer's inquiry, our CBLB antibody (NBP1-49919) has been validated for IHC and it is not recommended to use this product for western blot. The negative mark (-) indicates this product should not be used for western blot. I am sorry if this has caused confusion for your customers.