CBX3 Antibody - BSA Free
Novus Biologicals | Catalog # NB100-41429
![Immunohistochemistry: CBX3 Antibody [NB100-41429]](https://resources.rndsystems.com/images/products/CBX3-Antibody-Immunohistochemistry-NB100-41429-img0014.jpg "Immunohistochemistry: CBX3 Antibody [NB100-41429]")
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Key Product Details
Validated by
Independent Antibodies
Species Reactivity
Human, Mouse
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot (Negative)
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
The immunogen recognized by this antibody maps to a region between residue 133 and the C-terminus (residue 183) of human chromobox homolog 3 using the numbering given in entry NP_009207.2 (GeneID 11335).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
This antibody can be used as the primary antibody in a PLA assay with the following as complementing antibodies:NB100-322, NB100-381, NB100-2350
Scientific Data Images for CBX3 Antibody - BSA Free
Immunohistochemistry: CBX3 Antibody [NB100-41429]
Immunohistochemistry: CBX3 Antibody [NB100-41429] - Sample: FFPE section of human ovarian carcinoma (left) and mouse teratoma (right). Antibody: Affinity purified rabbit anti-CBX3 used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB staining using Immunohistochemistry Accessory Kit.![Immunoprecipitation: CBX3 Antibody [NB100-41429]](https://resources.rndsystems.com/images/products/CBX3-Antibody-Immunoprecipitation-NB100-41429-img0001.jpg "Immunoprecipitation: CBX3 Antibody [NB100-41429]")
Immunoprecipitation: CBX3 Antibody [NB100-41429]
Immunoprecipitation: CBX3 Antibody [NB100-41429] - Detection of Human CBX3 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-CBX3 antibody NB100-41429 used for IP at 3 mcg/mg lysate. CBX3 was also immunoprecipitated by rabbit anti-CBX3 antibodies NB100-41427 and NB100-41428, which recognize upstream epitopes. Detection: Chemiluminescence with an exposure time of 30 seconds.Applications for CBX3 Antibody - BSA Free
Application
Recommended Usage
Immunohistochemistry
1:10-1:500
Immunohistochemistry-Paraffin
1:10-1:500
Immunoprecipitation
2-5 ug/mg lysate
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: CBX3
Alternate Names
chromobox homolog 3, chromobox homolog 3 (Drosophila HP1 gamma), chromobox protein homolog 3, Drosophila), HECH, Heterochromatin protein 1 homolog gamma, heterochromatin protein HP1 gamma, heterochromatin-like protein 1, HP1 gamma, HP1 gamma homolog, HP1-GAMMA, Modifier 2 protein
Entrez Gene IDs
11335 (Human)
Gene Symbol
CBX3
UniProt
Additional CBX3 Products
Product Documents for CBX3 Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for CBX3 Antibody - BSA Free
This antibody can be used as the primary antibody in a PLA assay with the following as complementing antibodies:NB100-322, NB100-381, NB100-2350
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Citations for CBX3 Antibody - BSA Free
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Immunoprecipitation Protocol
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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