CD11c Antibody (CL1831) - BSA Free

Novus Biologicals | Catalog # NBP2-34491

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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Multiplex Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, COMET

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL1831

Format

BSA Free
View all CD11c Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed using a recombinant protein derived from P20702, with the exact immunogen sequence remaining proprietary.

Reactivity Notes

Mouse reactivity reported from a verified customer review.

Marker

Dendritic Cell Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for CD11c Antibody (CL1831) - BSA Free

CD11c Antibody (CL1831) - BSA Free CD11c Antibody (CL1831)

Detection of CD11c in Mouse Spleen via seqIF™ staining on COMET™​

CD11c was detected in immersion fixed paraffin-embedded sections of mouse Spleen using Mouse anti-CD11c, Monoclonal Antibody (Catalog #NBP2-34491) at 1:200 dilution at 37 ° Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9; Epredia Catalog # TA-999-DHBH). Tissue was stained using the Alexa Fluor™ 647 Goat anti-Mouse IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR647MS) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the cytoplasm. Protocol available in COMET™ Panel Builder.
Immunohistochemistry-Paraffin: CD11c Antibody (CL1831) [NBP2-34491]

Immunohistochemistry-Paraffin: CD11c Antibody (CL1831) [NBP2-34491]

Immunohistochemistry-Paraffin: CD11c Antibody (CL1831) [NBP2-34491] - Staining in human tonsil and kidney tissues. Corresponding ITGAX RNA-seq data are presented for the same tissues.
Western Blot: CD11c Antibody (CL1831) [NBP2-34491]

Western Blot: CD11c Antibody (CL1831) [NBP2-34491]

Western Blot: CD11c Antibody (CL1831) [NBP2-34491] - Lane 1: Marker [kDa]. Lane 2: Human tonsil.
Immunohistochemistry-Paraffin: CD11c Antibody (CL1831) [NBP2-34491]

Immunohistochemistry-Paraffin: CD11c Antibody (CL1831) [NBP2-34491]

Immunohistochemistry-Paraffin: CD11c Antibody (CL1831) [NBP2-34491] - CD11c Positive cells in mouse liver tissue. Primary antibody dilution: 1:100. Citrate buffer antigen retrieval. Overnight incubation RT. Image from verified customer review.
Immunohistochemistry-Paraffin: CD11c Antibody (CL1831) [NBP2-34491]

Immunohistochemistry-Paraffin: CD11c Antibody (CL1831) [NBP2-34491]

Immunohistochemistry-Paraffin: CD11c Antibody (CL1831) [NBP2-34491] - Staining of human tonsil shows strong membranous positivity in a subset of germinal and non-germinal center cells.
Immunohistochemistry-Paraffin: CD11c Antibody (CL1831) [NBP2-34491]

Immunohistochemistry-Paraffin: CD11c Antibody (CL1831) [NBP2-34491]

Immunohistochemistry-Paraffin: CD11c Antibody (CL1831) [NBP2-34491] - Staining of human small intestine shows moderate positivity in a subset of lymphoid cells.
Immunohistochemistry-Paraffin: CD11c Antibody (CL1831) [NBP2-34491]

Immunohistochemistry-Paraffin: CD11c Antibody (CL1831) [NBP2-34491]

Immunohistochemistry-Paraffin: CD11c Antibody (CL1831) [NBP2-34491] - Staining of human fallopian tube shows moderate membranous positivity in a subset of lymphoid cells.
Immunohistochemistry-Paraffin: CD11c Antibody (CL1831) [NBP2-34491]

Immunohistochemistry-Paraffin: CD11c Antibody (CL1831) [NBP2-34491]

Immunohistochemistry-Paraffin: CD11c Antibody (CL1831) [NBP2-34491] - Staining of human kidney shows no positivity as expected.

Applications for CD11c Antibody (CL1831) - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Reviewed Applications

Read 1 review rated 4 using NBP2-34491 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CD11c

CD11c encodes the integrin alpha X chain protein. CD11c is a type I transmembrane protein found at high levels on most human dendritic cells, but also on monocytes, macrophages, neutrophils, and some B cells that induces cellular activation and helps trigger neutrophil respiratory burst; expressed in hairy cell leukemias, acute nonlymphocytic leukemias, and some B-cell chronic lymphocytic leukemias.

Alternate Names

CD11c, Integrin alpha X, ITGAX, p150,95 alpha

Gene Symbol

ITGAX

UniProt

P20702 (Human)

Additional CD11c Products

Product Documents for CD11c Antibody (CL1831) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for CD11c Antibody (CL1831) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for CD11c Antibody (CL1831) - BSA Free (1)

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  • CD11c Antibody (CL1831)
    Name: Mozhdeh Sojoodi
    Application: Immunohistochemistry-Paraffin
    Sample Tested: Liver and Liver tissue
    Species: Mouse
    Verified Customer | Posted 02/07/2022
    Cd11c Positive cells in mouse liver
    1:100 dilution Citrate buffer antigen retrieval Overnight incubation RT
    CD11c Antibody (CL1831) - BSA Free NBP2-34491
    Bio-Techne Response
    This review was submitted through the legacy Novus Innovators Program, reflecting a new species or application tested on a primary antibody.

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Protocols

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