CD21 Antibody (SC0681)

Novus Biologicals | Catalog # NBP2-67605

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse, Monkey

Cited:

Primate - Macaca mulatta (Rhesus Macaque)

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, In-situ Hybridization

Cited:

In Situ Hybridization

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # SC0681 expressed in HEK293
View all CD21 Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptide within Human CD21 aa 989-1033 / 1033. (SwissProt: P20023 Human; SwissProt: P19070 Mouse)

Reactivity Notes

Use in Monkey reported in scientific literature (PMID:34855915).

Localization

Membrane.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for CD21 Antibody (SC0681)

Western Blot: CD21 Antibody (SC0681) [NBP2-67605]

Western Blot: CD21 Antibody (SC0681) [NBP2-67605]

Western Blot: CD21 Antibody (SC0681) [NBP2-67605] - Analysis of CD21 on Daudi celllysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody ( 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Immunocytochemistry/ Immunofluorescence: CD21 Antibody (SC0681) [NBP2-67605]

Immunocytochemistry/ Immunofluorescence: CD21 Antibody (SC0681) [NBP2-67605]

Immunocytochemistry/Immunofluorescence: CD21 Antibody (SC0681) [NBP2-67605] - Staining CD21 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunohistochemistry-Paraffin: CD21 Antibody (SC0681) [NBP2-67605]

Immunohistochemistry-Paraffin: CD21 Antibody (SC0681) [NBP2-67605]

Immunohistochemistry-Paraffin: CD21 Antibody (SC0681) [NBP2-67605] - Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-CD21 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1610-61, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunocytochemistry/ Immunofluorescence: CD21 Antibody (SC0681) [NBP2-67605]

Immunocytochemistry/ Immunofluorescence: CD21 Antibody (SC0681) [NBP2-67605]

Immunocytochemistry/Immunofluorescence: CD21 Antibody (SC0681) [NBP2-67605] - Staining CD21 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunohistochemistry-Paraffin: CD21 Antibody (SC0681) [NBP2-67605]

Immunohistochemistry-Paraffin: CD21 Antibody (SC0681) [NBP2-67605]

Immunohistochemistry-Paraffin: CD21 Antibody (SC0681) [NBP2-67605] - Analysis of paraffin-embedded human gastric cancer tissue using anti-CD21 antibody. Counter stained with hematoxylin.
Immunohistochemistry-Paraffin: CD21 Antibody (SC0681) [NBP2-67605]

Immunohistochemistry-Paraffin: CD21 Antibody (SC0681) [NBP2-67605]

Immunohistochemistry-Paraffin: CD21 Antibody (SC0681) [NBP2-67605] - Analysis of paraffin-embedded human spleen tissue using anti-CD21 antibody. Counter stained with hematoxylin.
Immunohistochemistry-Paraffin: CD21 Antibody (SC0681) [NBP2-67605]

Immunohistochemistry-Paraffin: CD21 Antibody (SC0681) [NBP2-67605]

Immunohistochemistry-Paraffin: CD21 Antibody (SC0681) [NBP2-67605] - Analysis of paraffin-embedded human tonsil tissue using anti-CD21 antibody. Counter stained with hematoxylin.
Immunohistochemistry-Paraffin: CD21 Antibody (SC0681) [NBP2-67605]

Immunohistochemistry-Paraffin: CD21 Antibody (SC0681) [NBP2-67605]

Immunohistochemistry-Paraffin: CD21 Antibody (SC0681) [NBP2-67605] - Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-CD21 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1610-61, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
CD21 Antibody (SC0681) Western Blot: CD21 Antibody (SC0681) [NBP2-67605]

Western Blot: CD21 Antibody (SC0681) [NBP2-67605]

Western blot analysis on different lysates with NBP2-67605 at 1/5,000 dilution. Lane 1: Raji cell lysate Lane 2: K-562 cell lysate (negative) Lysates/proteins at 20 µg/Lane. Predicted band size: 113 kDa Observed band size: 150 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP2-67605 at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG [HRP] Secondary Antibody at 1:50,000 dilution was used for 1 hour at room temperature.

Applications for CD21 Antibody (SC0681)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50-1:200

Immunohistochemistry-Paraffin

1:50-1:800

Western Blot

1:1000-1:2000
Application Notes
Use in In-situ Hybridization reported in scientific literature (PMID:34855915)..

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

TBS (pH7.4), 0.05% BSA, 40% Glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CD21

CD21 (complement receptor 2, CR2) binds C3 complement fragments, especially its breakdown fragments, which remain covalently attached to complement activating surfaces or antigen. CD21 has important roles in uptake and retention of immunocomplexes, survival of memory B cells and in development and maintenance of the humoral response to T-dependent antigens. CD21 also serves as a key receptor for Epstein-Barr virus binding and is involved in targeting prions to folicular dendritic cells and expediting neuroinvasion following peripheral exposure to prions. A soluble form of the CD21 (sCD21) is shed from the lymphocyte surface and retains its ability to bind respective ligands.

Alternate Names

C3DR, CD21, CR2

Gene Symbol

CR2

Additional CD21 Products

Product Documents for CD21 Antibody (SC0681)

Certificate of Analysis

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Product Specific Notices for CD21 Antibody (SC0681)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for CD21 Antibody (SC0681)

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