Key Product Details

Species Reactivity

Validated:

Rat

Cited:

Human, Mouse, Rat

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Flow Cytometry, Flow (Intracellular), Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Cited:

Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, IF/IHC

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # ED1

Format

BSA Free
View all CD68/SR-D1 Primary Antibodies »

Product Specifications

Immunogen

Rat spleen cells

Reactivity Notes

Predicted cross-reactivities: Bovine

Marker

Macrophage Marker

Specificity

This CD68/SR-D1 Antibody (ED1) recognizes a single chain glycoprotein of 110 kDa that is expressed predominantly on the lysosomal membrane of myeloid cells. Weak cell surface expression also occurs. The antigen is expressed by the majority of tissue macrophages and weakly by peripheral blood granulocytes. Studies have shown that the antigen recognized by ED1 is the rat homologue of human CD68.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for CD68/SR-D1 Antibody (ED1) - BSA Free

Immunohistochemistry-Paraffin: CD68/SR-D1 Antibody (ED1) - BSA Free [NB600-985]

Immunohistochemistry-Paraffin: CD68/SR-D1 Antibody (ED1) - BSA Free [NB600-985]

Immunohistochemistry-Paraffin: CD68/SR-D1 Antibody (ED1) [NB600-985] - Cynomolgus monkey spleen. IHC-P image submitted by a verified customer review.
Flow Cytometry: CD68/SR-D1 Antibody (ED1) - BSA Free [NB600-985]

Flow Cytometry: CD68/SR-D1 Antibody (ED1) - BSA Free [NB600-985]

Flow Cytometry: CD68/SR-D1 Antibody (ED1) [NB600-985] - Staining of rat peritoneal macrophages cells.
Flow Cytometry: CD68/SR-D1 Antibody (ED1) - BSA Free [NB600-985]

Flow Cytometry: CD68/SR-D1 Antibody (ED1) - BSA Free [NB600-985]

Flow Cytometry: CD68/SR-D1 Antibody (ED1) [NB600-985] - Staining of rat peritoneal macrophages.
Flow Cytometry: CD68/SR-D1 Antibody (ED1) - BSA Free [NB600-985]

Flow Cytometry: CD68/SR-D1 Antibody (ED1) - BSA Free [NB600-985]

Flow Cytometry: CD68/SR-D1 Antibody (ED1) [NB600-985] - Staining of rat peritoneal macrophages
Immunohistochemistry-Frozen: CD68/SR-D1 Antibody (ED1) - BSA Free [NB600-985]

Immunohistochemistry-Frozen: CD68/SR-D1 Antibody (ED1) - BSA Free [NB600-985]

Immunohistochemistry-Frozen: CD68/SR-D1 Antibody (ED1) [NB600-985] - The feline macrophage containing section was incubated with the antibody at 1:50 and the section was further stained with Alexa secondary antibody. Image was captured with an epifluorescence microscope. IHC-Fr image submitted by a verified customer review.
Flow Cytometry: CD68/SR-D1 Antibody (ED1) - BSA Free [NB600-985]

Flow Cytometry: CD68/SR-D1 Antibody (ED1) - BSA Free [NB600-985]

Flow Cytometry: CD68/SR-D1 Antibody (ED1) [NB600-985] - Decidual Macrophages were analyzed with a BD LSRFortessa. Antibody was diluted 1:100 in staining buffer before use. Isotype control is in Red. Sample with NB600-985AF700 is in Blue. Image from the Alexa Fluor 700 version of this antibody.
Immunohistochemistry-Paraffin: CD68/SR-D1 Antibody (ED1) - BSA Free [NB600-985]

Immunohistochemistry-Paraffin: CD68/SR-D1 Antibody (ED1) - BSA Free [NB600-985]

Immunohistochemistry-Paraffin: CD68/SR-D1 Antibody (ED1) [NB600-985] - Staining of rat liver
Flow Cytometry: CD68/SR-D1 Antibody (ED1) - BSA Free [NB600-985]

Flow Cytometry: CD68/SR-D1 Antibody (ED1) - BSA Free [NB600-985]

Flow Cytometry: CD68/SR-D1 Antibody (ED1) [NB600-985] - Staining of rat peritoneal macrophages cells.
Flow Cytometry: CD68/SR-D1 Antibody (ED1) - BSA Free [NB600-985]

Flow Cytometry: CD68/SR-D1 Antibody (ED1) - BSA Free [NB600-985]

Flow Cytometry: CD68/SR-D1 Antibody (ED1) [NB600-985] - An intracellular stain was performed on Jurkat cells with BAK antibody (8B4) NBP1-74026PE (blue) and a matched isotype control NB600-985PE (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to phycoerythrin. Image using the PE form of this antibody.
Flow (Intracellular): CD68/SR-D1 Antibody (ED1) - BSA Free [NB600-985]

Flow (Intracellular): CD68/SR-D1 Antibody (ED1) - BSA Free [NB600-985]

Flow (Intracellular): CD68/SR-D1 Antibody (ED1) [NB600-985] - An intracellular stain was performed on U-937 cells with CD68/SR-D1 Antibody (ED1) NB600-985PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to phycoerythrin.

Applications for CD68/SR-D1 Antibody (ED1) - BSA Free

Application
Recommended Usage

Flow Cytometry

1:50 - 1:100

Immunocytochemistry/ Immunofluorescence

1:10 - 1:500

Immunohistochemistry

1:10 - 1:500

Immunohistochemistry-Frozen

1:10 - 1:500

Immunohistochemistry-Paraffin

1:100

Immunoprecipitation

1:10 - 1:500

Western Blot

1:100 - 1:2000
Application Notes
Flow Cytometry: Use 10 uL of the suggested working dilution (1:50 - 1:100) to label 10^6 cells in 100 uL. Membrane permeabilization is required for Flow Cytometry. Immunohistology on frozen and paraffin sections: Use at a dilution of 1:100, requires protein digestion pretreatment with pronase or trypsin. WB: Use at an assay dependent dilution. IP: Use at an assay dependent dilution. RIA: Use at an assay dependent dilution.

Reviewed Applications

Read 2 reviews rated 4.5 using NB600-985 in the following applications:

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CD68/SR-D1

Human CD68, also known as GP110, LAMP4, Scavenger Receptor D1 (SR-D1) or macrosialin in mouse, encodes a 110-kD transmembrane glycoprotein that belongs to the lysosomal/endosomal-associated membrane glycoprotein (LAMP) family. Members of the LAMP family include LAMP-1, LAMP-2, dendritic cell (DC)-LAMP (aka CD208), and brain and dendritic cell-associated (BAD)-LAMP (aka LAMP-5). Unlike the two LAMP domains facing the lysosomal lumen in LAMP-1 and LAMP-2, CD68 has a single LAMP domain containing four cystines spaced 36-37 residues apart along with an N-terminal Mucin-like domain. The 354 amino acid (a.a.) human CD68 and 326 a.a. murine ortholog share 80.6% a.a. sequence identity (1).

CD68 is highly expressed in cells of the mononuclear phagocyte system such as macrophages, microglia, osteoclasts, and myeloid dendritic cells (DCs); and is expressed to a lesser extent in lymphoid cells (CD19+ B lymphocytes and CD4+ T lymphocytes), human umbilical cord mesenchymal stem cells (MSCs), fibroblasts, endothelial cells, multiple non-hematopoietic cancer cell lines, and human arterial intimal smooth muscle cells (SMCs). Expression has been also observed in diseased states for granulocytes and neutrophils, in particular basophils from myeloproliferative disorders and intestinal neutrophils from inflammatory bowel disease (IBD), respectively (1).

Although the function of CD68 has yet to be established, it has often been used as an immunohistochemistry (IHC) marker of inflammation and for granular cell tumors (GCTs). CD68+ tumor associated macrophages (TAMs) has been suggested to be a predictive marker for poor cancer prognosis, but a meta-analysis showed the presence of CD68 is not correlated with survival (2). In addition, a role in hepatic malaria infection has been reported based on the finding that peptide P39 binds CD68, considered a receptor for malaria sporozoite, and inhibits parasite entry into Kupffer cells. CD68 was deemed a member of the Scavenger receptor family due to its upregulation in macrophages following inflammatory stimuli, ability to bind modified LDL, phosphatidylserine, and apoptotic cells, as well as shuttling between the plasma membrane and endosomes. CD68 has been linked to atherogenesis based on binding and internalization of its ligand, oxLDL (1).

References

1. Chistiakov, DA, Killingsworth, MC, Myasoedova, VA. Orekhov AN, Bobryshev YV. (2017) CD68/macrosialin: not just a histochemical marker. Lab Invest. 97:4-13. PMID: 27869795

2. Troiano G, Caponio VCA, Adipietro I, Tepedino M, Santoro R, Laino L, Lo Russo L, Cirillo N, Lo Muzio L. (2019) Prognostic significance of CD68+ and CD163+ tumor associated macrophages in head and neck squamous cell carcinoma: A systematic review and meta-analysis. Oral Oncol. 93:66-75. PMID: 31109698.

Alternate Names

CD68, gp110, Macrosialin, SCARD1, SR-D1, SRD1

Entrez Gene IDs

287435 (Rat)

Gene Symbol

CD68

UniProt

Q4FZY1 (Rat)

Additional CD68/SR-D1 Products

Product Documents for CD68/SR-D1 Antibody (ED1) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for CD68/SR-D1 Antibody (ED1) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for CD68/SR-D1 Antibody (ED1) - BSA Free

Customer Reviews for CD68/SR-D1 Antibody (ED1) - BSA Free (2)

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Customer Images

Showing  1 - 2 of 2 reviews Showing All
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  • CD68/SR-D1 Antibody (ED1)
    Name: Anonymous
    Application: Immunohistochemistry-Frozen
    Sample Tested: macrophage
    Species: Feline
    Verified Customer | Posted 03/09/2018
    The feline macrophage containing section was incubated with the antibody at 1:50 and the section was further stained with Alexa secondary antibody. Image was captured with an epifluorescence microscope.
    CD68/SR-D1 Antibody (ED1) - BSA Free NB600-985
  • Name: Anonymous
    Application: Immunohistochemistry-Paraffin
    Sample Tested: cynomolgus monkey spleen
    Species: Other
    Verified Customer | Posted 06/02/2014
    cynomolgus monkey spleen
    CD68/SR-D1 Antibody (ED1) - BSA Free NB600-985

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