CEBP epsilon Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-85446

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Cited:

Human, Mouse

Predicted:

Rat (94%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Chromatin Immunoprecipitation-exo-Seq

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all CEBP epsilon Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: SHGTYYECEPRGGQQPLEFSGGRAGPGELGDMCEHEASIDLSAYIESGEEQLLSDLFAVKPAPEARGLKGPGTPAFPHYLPPDPRPFAYPPHTFGPDRKALGPGIYSSPGSYDPRAVAVKEEPR

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID:31912614).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for CEBP epsilon Antibody - BSA Free

Immunohistochemistry-Paraffin: CEBP epsilon Antibody [NBP1-85446]

Immunohistochemistry-Paraffin: CEBP epsilon Antibody [NBP1-85446]

Immunohistochemistry-Paraffin: CEBP epsilon Antibody [NBP1-85446] - Staining in human bone marrow and cerebral cortex tissues using anti-CEBPE antibody. Corresponding CEBPE RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: CEBP epsilon Antibody [NBP1-85446]

Immunohistochemistry-Paraffin: CEBP epsilon Antibody [NBP1-85446]

Immunohistochemistry-Paraffin: CEBP epsilon Antibody [NBP1-85446] - Staining of human bone marrow shows high expression.
Immunohistochemistry-Paraffin: CEBP epsilon Antibody [NBP1-85446]

Immunohistochemistry-Paraffin: CEBP epsilon Antibody [NBP1-85446]

Immunohistochemistry-Paraffin: CEBP epsilon Antibody [NBP1-85446] - Staining of human cerebral cortex shows low expression as expected.
CEBP epsilon Antibody - BSA Free Chromatin Immunoprecipitation-exo-Seq: CEBP epsilon Antibody - BSA Free [NBP1-85446]

Chromatin Immunoprecipitation-exo-Seq: CEBP epsilon Antibody - BSA Free [NBP1-85446]

ChIP-Exo-Seq composite graph for Anti-CEBPE (NBP1-85446) tested in K562 cells. Strand-specific reads (blue: forward, red: reverse) and IgG controls (black: forward, grey: reverse) are plotted against the distance from a composite set of reference binding sites. The antibody exhibits robust target enrichment compared to a non-specific IgG control and precisely reveals its structural organization around the binding site. Data generated by Prof. B. F. Pugh´s Lab at Cornell University.
CEBP epsilon Antibody - BSA Free

CEBP epsilon Antibody [NBP1-85446] -

Analysis in human cell line HL-60.
CEBP epsilon Antibody - BSA Free

CEBP epsilon Antibody [NBP1-85446] -

Staining of human skeletal muscle shows no positivity in myocytes as expected.
CEBP epsilon Antibody - BSA Free Immunocytochemistry/ Immunofluorescence: CEBP epsilon Antibody [NBP1-85446]

Immunocytochemistry/ Immunofluorescence: CEBP epsilon Antibody [NBP1-85446]

Staining of human cell line U-251 MG shows localization to nucleoplasm.

Applications for CEBP epsilon Antibody - BSA Free

Application
Recommended Usage

Chromatin Immunoprecipitation-exo-Seq

1-10ug per reaction

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CEBP epsilon

EBP1 (ErbB-3-binding protein 1), also known as PA2G4 (proliferation-associated 2G4), p38-2G4 or HG4-1, is a member of the peptidase M24C family and functions as an RNA-binding protein involved in cellular proliferation and differentiation processes. It is expressed in a variety of cell lines, including a wide range of tumor cell lines, and localizes to the cytoplasm. Upon treatment with Neuregulin-1 (heregulin), EBP1 translocates to the nucleus. EBP1 is a component of pre-ribosomal ribonucleoprotein complexes, participating in ribosome assembly and regulating the later steps of rRNA processing. In addition, EBP1 interacts with ErbB-3 and may function as a modulator of the ErbB-3-mediated signal transduction pathway by regulating the effects of Neuregulin-1. EBP1 also associates with histone deacetylases (HDACs), functioning as a transcriptional corepressor of cell cycle regulatory genes

Long Name

CCAAT/enhancer-Binding Protein epsilon

Alternate Names

CEBPE

Gene Symbol

CEBPE

Additional CEBP epsilon Products

Product Documents for CEBP epsilon Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for CEBP epsilon Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for CEBP epsilon Antibody - BSA Free

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Protocols

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