CHD1 Antibody

Chromatin Immunoprecipitation: CHD1 Antibody [NB100-60411] - ChIP-chip scatter plot of anti-CHD1 (NB100-60411) enriched DNA binding sites versus input reference DNA. CHD1 was used to immunoprecipitate chromatin from K562 cells according to Ren et al (Genes Dev. 2002 16: 245-256). Immunoprecipitated DNA and reference DNA were amplified via ligation-mediated PCR and the products labeled with fluorescent dUTPs. The labeled ChIP and reference DNA were pooled, hybridized to a DNA microarray, and analyzed. Data points below the +3 SD curve (red line) represent significantly enriched binding sites. B. As a control, a similar experiment was performed using normal rabbit IgG. Compared to the anti-CHD1 ChIP, normal rabbit IgG showed little enrichment.

Immunocytochemistry/ Immunofluorescence: CHD1 Antibody [NB100-60411]

CHD1-Antibody-Knockdown-Validated-NB100-60411-img0008.jpg

Western Blot: CHD1 Antibody [NB100-60411] -

Chromatin marks associated with transcriptional elongation are increased in the absence of Lsh.(A) Schematic diagram depicting the position of the primer pairs (black lines) used for ChIP analysis. Comparison of the ratio of H3K36Me3 (B), H3K79Me2 (C) and H3K4Me3 (E) enrichment to total H3 occupancies (Fig. S6) between Lsh WT and Lsh−/−MEFs at up, TSS, down and 3′UTR regions of HoxC6 and HoxC8 genes. (D) ChIP analysis for the detection of Ser 2 RNA Pol II (H5). (F) ChIP analysis for detection of Chd1. (G) Western analysis for detection of Chd1 protein comparing whole cell extracts derived from Lsh−/−MEFs targeted by Chd1 siRNA or control siRNA (H) Real-time RT-PCR analysis detecting HoxC6 and HoxC8 mRNA in Lsh−/− MEFs after targeting by Chd1 siRNA compared to control treated cells. Results represent standard deviations for the mean of three independent experiments. Asterix indicates a p value p<0.05. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/20161795), licensed under a CC0-1.0 license. Not internally tested by Novus Biologicals.