Chk2 Antibody (24GB4585)

Novus Biologicals | Catalog # NBP3-51662

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Knockdown Validated

Label

Unconjugated

Antibody Source

Monoclonal Rabbit IgG Clone # 24GB4585
View all Chk2 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein of human Chk2 (Uniprot: O96017)

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Chk2 Antibody (24GB4585)

Chk2 Antibody (24GB4585)

Immunocytochemistry/ Immunofluorescence: Chk2 Antibody (24GB4585)[NBP3-51662] -

Chk2 antibody (1:1,000), Top panel: wild-type (WT); Bottom panal: Checkpoint kinase 2 shRNA knockdown (KD). Nuclei were stained blue with DAPI; Checkpoint kinase 2 was stained magenta with Alexa Fluor(R) 647. Scale bar, 20 um.
Chk2 Antibody (24GB4585)

Immunocytochemistry/ Immunofluorescence: Chk2 Antibody (24GB4585)[NBP3-51662] -

Immunocytochemical staining of HAP-1 cells with Chk2 antibody (1:1,000). Nuclei were stained blue with DAPI;Chk2 was stained magenta with Alexa Fluor(R) 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 um.
Chk2 Antibody (24GB4585)

Flow Cytometry: Chk2 Antibody (24GB4585)[NBP3-51662] -

Flow cytometric analysis of Chk2expression in HAP-1 cells using Chk2 antibody (1:2,000). Green, isotype control; red, Checkpoint kinase 2.
Chk2 Antibody (24GB4585)

Flow Cytometry: Chk2 Antibody (24GB4585)[NBP3-51662] -

Validation of Chk2 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with Chk2 antibody (1:2,000) and analyzed using BD flow cytometer.
Chk2 Antibody (24GB4585)

Western Blot: Chk2 Antibody (24GB4585)[NBP3-51662] -

Western blotting analysis using Chk2 antibody (1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using NaQ(TM) ECL Substrate Kit.

Applications for Chk2 Antibody (24GB4585)

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Knockdown Validated

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry Panel Builder

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Advanced Features

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.4), 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Chk2

In response to DNA damage and replication blocks, cell cycle progression is halted through the control of critical cell cycle regulators. The protein encoded by this gene is a cell cycle checkpoint regulator and putative tumor suppressor. It contains a forkhead-associated protein interaction domain essential for activation in response to DNA damage and is rapidly phosphorylated in response to replication blocks and DNA damage. When activated, the encoded protein is known to inhibit CDC25C phosphatase, preventing entry into mitosis, and has been shown to stabilize the tumor suppressor protein p53, leading to cell cycle arrest in G1. In addition, this protein interacts with and phosphorylates BRCA1, allowing BRCA1 to restore survival after DNA damage. Mutations in this gene have been linked with Li-Fraumeni syndrome, a highly penetrant familial cancer phenotype usually associated with inherited mutations in TP53. Also, mutations in this gene are thought to confer a predisposition to sarcomas, breast cancer, and brain tumors. This nuclear protein is a member of the CDS1 subfamily of serine/threonine protein kinases. Three transcript variants encoding different isoforms have been found for this gene.

Long Name

Checkpoint Kinase 2

Alternate Names

CDS1, CHEK2, HuCds1, LFS2, PP1425, Rad53

Gene Symbol

CHEK2

Additional Chk2 Products

Product Documents for Chk2 Antibody (24GB4585)

Certificate of Analysis

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Product Specific Notices for Chk2 Antibody (24GB4585)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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