Chloroquine Inhibitor

Novus Biologicals | Catalog # NBP2-29386

Novus Biologicals
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Key Product Details

Applications

Block/Neutralize

Product Specifications

Application Notes

DNA intercalator. Also used to increase transfection efficiency. Use in blocking/ neutralizing reported in scientific literature (PMID: 30239551).

Scientific Data Images for Chloroquine Inhibitor

Chloroquine Inhibitor [NBP2-29386] - TLR9/NF-kB/SEAPorter™ HEK 293 and TLR2/NF-kB/SEAPorter™ HEK 293 cells were plated in 96-well plates at 5x10^4 cells/well for 16 h. Cells were preincubated with different concentrations of chloroquine (0.05, 0.1, 0.5, 1, 5 and 10 uM) for 30 min. Cell were then stimulated with 10ug/ml CpG [A] or 20 ng/ml MALP-2 [B] for 24 h. Secreted alkaline phosphatase (SEAP) was analyzed using SEAPorter™ Assay Kit.
Chloroquine Inhibitor [NBP2-29386] - TLR9/NF-kB/SEAPorter™ HEK 293 cells were plated in 96-well plates at 5x10^4 cells/well for 16 h. Cells were preincubated with various concentrations of chloroquine for 30 min, followed by stimulation with 10 ug/ml CpG for 24 h. SEAP was analyzed using SEAPorter™ Assay Kit. Dose-responsive percent inhibition of each sample well was calculated to yield the chloroquine IC50 value.
Chloroquine Inhibitor [NBP2-29386]

Formulation, Preparation, and Storage

Formulation

Solubility:Deionized water
Form : White Solid (Powder)

Concentration

Concentration is not relevant for this product. Please see the protocols for proper use of this product.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store at 4C in the dark.

Background: Chloroquine

Chloroquine is a weak base which can partition into acidic vesicles such as endosomes and lysosomes, resulting in inhibition of endosomal acidification and lysosomal enzyme activity. Because acidic pH of endosomes is a prerequisite of endosomal TLR activation, chloroquine can serve as an antagonist for endosomal TLRs. Chloroquine and its analog quinacrine are also known to act as therapeutic agents for autoimmune diseases such as rheumatoid arthritis and systemic lupus erythematosus, of which therapeutic activity is due to suppression of TLR9 activity as shown by researchers.

Additional Chloroquine Products

Product Documents for Chloroquine Inhibitor

Certificate of Analysis

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Product Specific Notices for Chloroquine Inhibitor

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Inhibitors are guaranteed for 1 year from date of receipt.

Citations for Chloroquine Inhibitor

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Protocols

View specific protocols for Chloroquine Inhibitor (NBP2-29386):

Product Handling Protocol (NBP2-29386):
1. To make 100 mM stock solution, dissolve 200 mg chloroquine in 3.9 ml water by gentle vortex.
2. Filter sterilize through a 0.22 um filter.
3. Store at 4oC (Note: Chloroquine solution is light sensitive).
4. For TLR signaling inhibition study, perform a pilot inhibitory test with the different concentrations of chloroquine ranging from 1 to 10 mM to optimize your experiments.

FAQs for Chloroquine Inhibitor

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  • Q: I am looking to test the effect of blocking the TLR9 on animal model (pigs with sternotomy) by chloroquine, Can you kindly advice me regarding whether is it possible to try it in vivo for approximatley 50kg animal and what is the optimum dose for better effect aftrer few hours of adminstration of the dose?

    A:

    We evaluate Chloroquinone on TLR9 Stably Transfected cell lines, but have not evaluated Chloroquine in animals. However, Chloroquine has been widely published for in vivo use. I would encourage you to review the scientific literature regarding the use of Chloroquine in animals for inhibiting TLR9, for example please seee this LINK.

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