CIP2A Antibody (2G10) - Azide and BSA Free
Novus Biologicals | Catalog # NBP2-80670
![Western Blot: CIP2A Antibody (2G10)Azide and BSA Free [NBP2-80670]](https://resources.rndsystems.com/images/products/CIP2A-Antibody-2G10-Azide-and-BSA-Free-Western-Blot-NBP2-80670-img0002.jpg "Western Blot: CIP2A Antibody (2G10)Azide and BSA Free [NBP2-80670]")
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Key Product Details
Validated by
Knockout/Knockdown
Species Reactivity
Human, Mouse, Rat, Primate
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Knockdown Validated
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2b Kappa Clone # 2G10
Format
Azide and BSA Free
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Product Specifications
Immunogen
A synthetic peptide made to a C-terminal portion of the human CIP2A protein. [UniProt# Q8TCG1]
Epitope
A 300 amino acid portion of the C-terminus.
Reactivity Notes
Other species have not been tested.
Localization
Cytoplasmic
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2b Kappa
Scientific Data Images for CIP2A Antibody (2G10) - Azide and BSA Free
Western Blot: CIP2A Antibody (2G10)Azide and BSA Free [NBP2-80670]
Western Blot: CIP2A Antibody (2G10) - Azide and BSA Free [NBP2-80670] - Detection of CIP2A in HeLa whole cell lysate. Image from the standard format of this antibody.![Immunocytochemistry/ Immunofluorescence: CIP2A Antibody (2G10) - Azide and BSA Free [NBP2-80670]](https://resources.rndsystems.com/images/products/CIP2A-Antibody-2G10-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-80670-img0003.jpg "Immunocytochemistry/ Immunofluorescence: CIP2A Antibody (2G10) - Azide and BSA Free [NBP2-80670]")
Immunocytochemistry/ Immunofluorescence: CIP2A Antibody (2G10) - Azide and BSA Free [NBP2-80670]
Immunocytochemistry/Immunofluorescence: CIP2A Antibody (2G10) - Azide and BSA Free [NBP2-80670] - CIP2A antibody was tested in U2OS cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red). Image from the standard format of this antibody.![Immunohistochemistry-Paraffin: CIP2A Antibody (2G10) - Azide and BSA Free [NBP2-80670]](https://resources.rndsystems.com/images/products/CIP2A-Antibody-2G10-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-80670-img0004.jpg "Immunohistochemistry-Paraffin: CIP2A Antibody (2G10) - Azide and BSA Free [NBP2-80670]")
Immunohistochemistry-Paraffin: CIP2A Antibody (2G10) - Azide and BSA Free [NBP2-80670]
Immunohistochemistry-Paraffin: CIP2A Antibody (2G10) - Azide and BSA Free [NBP2-80670] - IHC analysis of formalin fixed paraffin-embedded (FFPE) human colon cancer using CIP2A antibody at 1:200 on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (![Flow Cytometry: CIP2A Antibody (2G10) - Azide and BSA Free [NBP2-80670]](https://resources.rndsystems.com/images/products/CIP2A-Antibody-2G10-Azide-and-BSA-Free-Flow-Cytometry-NBP2-80670-img0005.jpg "Flow Cytometry: CIP2A Antibody (2G10) - Azide and BSA Free [NBP2-80670]")
Flow Cytometry: CIP2A Antibody (2G10) - Azide and BSA Free [NBP2-80670]
Flow Cytometry: CIP2A Antibody (2G10) - Azide and BSA Free [NBP2-80670] - Intracellular flow cytometric staining of 1 x 10^6 CHO (A) and HEK-293 (B) cells using CIP2A antibody (dark blue). Isotype control shown in orange. An antibody concentration of 1 ug/1x10^6 cells was used. Image from the standard format of this antibody.![Western Blot: CIP2A Antibody (2G10)Azide and BSA Free [NBP2-80670]](https://resources.rndsystems.com/images/products/CIP2A-Antibody-2G10-Azide-and-BSA-Free-Western-Blot-NBP2-80670-img0001.jpg "Western Blot: CIP2A Antibody (2G10)Azide and BSA Free [NBP2-80670]")
Western Blot: CIP2A Antibody (2G10)Azide and BSA Free [NBP2-80670]
Western Blot: CIP2A Antibody (2G10) - Azide and BSA Free [NBP2-80670] - Analysis of CIP2A in NIH/3T3 cell lysate. Image from the standard format of this antibody.![Knockdown Validated: CIP2A Antibody (2G10) - Azide and BSA Free [NBP2-80670]](https://resources.rndsystems.com/images/products/CIP2A-Antibody-2G10-Azide-and-BSA-Free-Knockdown-Validated-NBP2-80670-img0006.jpg "Western Blot: CIP2A Antibody (2G10) - Azide and BSA Free [NBP2-80670]")
Western Blot: CIP2A Antibody (2G10) - Azide and BSA Free [NBP2-80670]
Western Blot: CIP2A Antibody (2G10) - Azide and BSA Free [NBP2-80670] - miR-375 increases p21, p53, and RB in HPV16- and 18-positive cancer. Protein levels of CIP2A, p53, and p21 in SiHa cells transfected with si-CIP2A and/or si-p53 were measured by Western blot analysis. Protein levels of CIP2A, p53, and p21 in SiHa cells transfected with si-p53 and/or miR-375-mimic were measured by Western blot analysis. Image collected and cropped by CiteAb from the following publication (https://molecular-cancer.biomedcentral.com/articles/10.1186/1476-4598-1…) licensed under a CC-BY license. Image from the standard format of this antibody.Applications for CIP2A Antibody (2G10) - Azide and BSA Free
Application
Recommended Usage
Flow Cytometry
1 ug per million cells
Immunocytochemistry/ Immunofluorescence
1:40
Immunohistochemistry
1:100 - 1:200
Immunohistochemistry-Paraffin
reported in scientific literature (PMID 25965834)
Western Blot
1:1000-1:2000
Application Notes
In ICC/IF, strong cytoplasmic staining is observed in U20s cells. In Western Blot analysis, a band is seen at ~90 kDa representing CIP2A.
Flow Cytometry Panel Builder
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Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
Tris-Glycine, 0.15 M NaCl
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: CIP2A
Alternate Names
Cancerous inhibitor of PP2A, CIP2A, FLJ12850, KIAA1524, MGC163436, p90, p90 autoantigen, protein CIP2A
Gene Symbol
CIP2A
Additional CIP2A Products
Product Documents for CIP2A Antibody (2G10) - Azide and BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for CIP2A Antibody (2G10) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- 7-Amino Actinomycin D (7-AAD) Cell Viability Flow Cytometry Protocol
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Extracellular Membrane Flow Cytometry Protocol
- Flow Cytometry Protocol for Cell Surface Markers
- Flow Cytometry Protocol for Staining Membrane Associated Proteins
- Flow Cytometry Staining Protocols
- Flow Cytometry Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Intracellular Flow Cytometry Protocol Using Alcohol (Methanol)
- Intracellular Flow Cytometry Protocol Using Detergents
- Intracellular Nuclear Staining Flow Cytometry Protocol Using Detergents
- Intracellular Staining Flow Cytometry Protocol Using Alcohol Permeabilization
- Intracellular Staining Flow Cytometry Protocol Using Detergents to Permeabilize Cells
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Propidium Iodide Cell Viability Flow Cytometry Protocol
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Liperfluo
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Characterization of Human Th22 Cells
- Protocol for the Characterization of Human Th9 Cells
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- Protocol: Annexin V and PI Staining by Flow Cytometry
- Protocol: Annexin V and PI Staining for Apoptosis by Flow Cytometry
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Fluorokine Flow Cytometry Kits
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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