CLCN2 Antibody (3E1) - Azide and BSA Free
Novus Biologicals | Catalog # H00001181-M01
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Key Product Details
Species Reactivity
Human
Applications
Western Blot, ELISA, Sandwich ELISA, Knockdown Validated
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2a Kappa Clone # 3E1
Format
Azide and BSA Free
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Product Specifications
Immunogen
CLCN2 (AAH21578.1, 38 a.a. ~ 387 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. TVSTAVIVFELTGQIAHILPVMIVVILANAVAQSLQPSLYDSIIRIKKLPYLPELGWGRHQQYRVRVEDIMVRDVPHVALSCTFRDLRLALHRTKGRMLALVESPESMILLGSIERSQVVALLGAQLSPARRRQHMQERRATQTSPLSDQEGPPTPEASVCFQVNTEDSAFPAARGETHKPLKPALKRGPSVTRNLGESPTGSAESAGIALRSLFCGSPPPEAASEKLESCEKRKLKRVRISLASDADLEGEMSPEETHTIFSLLGVDHAYVTSIGRLIGIVTLKELRKAIEGSVTAQGVKVRPPLASFRDSATSSSDTETTEVHALWGPHSRHGLPREGSPSDSDDKCQ
Specificity
CLCN2 - chloride channel 2
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2a Kappa
Description
Quality control test: Antibody Reactive Against Recombinant Protein.
Scientific Data Images for CLCN2 Antibody (3E1) - Azide and BSA Free
Western Blot: CLCN2 Antibody (3E1) [H00001181-M01]
Western Blot: CLCN2 Antibody (3E1) [H00001181-M01] - Analysis of CLCN2 expression in transfected 293T cell line by CLCN2 monoclonal antibody (M01), clone 3E1.Lane 1: CLCN2 transfected lysate(96.8 KDa).Lane 2: Non-transfected lysate.Western Blot: CLCN2 Antibody (3E1) [H00001181-M01]
Western Blot: CLCN2 Antibody (3E1) [H00001181-M01] - Analysis of CLCN2 over-expressed 293 cell line, cotransfected with CLCN2 Validated Chimera RNAi ( Cat # H00001181-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with CLCN2 monoclonal antibody (M01), clone 3E1 (Cat # H00001181-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.Western Blot: CLCN2 Antibody (3E1) [H00001181-M01]
Western Blot: CLCN2 Antibody (3E1) [H00001181-M01] - CLCN2 monoclonal antibody (M01), clone 3E1 Analysis of CLCN2 expression in Hela S3 NE.
Sandwich ELISA: CLCN2 Antibody (3E1) [H00001181-M01] - Detection limit for recombinant GST tagged CLCN2 is 0.3 ng/ml as a capture antibody.
Applications for CLCN2 Antibody (3E1) - Azide and BSA Free
Application
Recommended Usage
Western Blot
1:500
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for ELISA and RNAi Validation.
Formulation, Preparation, and Storage
Purification
IgG purified
Formulation
In 1x PBS, pH 7.4
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: CLCN2
Long Name
Chloride channel protein 2
Alternate Names
ClC-2
Entrez Gene IDs
1181 (Human)
Gene Symbol
CLCN2
OMIM
600570 (Human)
UniProt
Additional CLCN2 Products
Product Documents for CLCN2 Antibody (3E1) - Azide and BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for CLCN2 Antibody (3E1) - Azide and BSA Free
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
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- How to Run an R&D Systems Quantikine ELISA
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- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- R&D Systems Quality Control Western Blot Protocol
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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