Cleavage stimulation factor 2 Antibody
Novus Biologicals | Catalog # NB100-57531
![Western Blot: Cleavage stimulation factor 2 Antibody [NB100-57531]](https://resources.rndsystems.com/images/products/Cleavage-stimulation-factor-2-Antibody-Western-Blot-NB100-57531-img0006.jpg "Western Blot: Cleavage stimulation factor 2 Antibody [NB100-57531]")
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Key Product Details
Species Reactivity
Human, Mouse
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunoprecipitation
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
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Product Specifications
Immunogen
The immunogen recognized by this antibody maps to a region between residue 527 and 577 of human cleavage stimulation factor, 3' pre-RNA, subunit 2, 64kDa using the numbering given in entry NP_001316.1 (GeneID 1478).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Cleavage stimulation factor 2 Antibody
Western Blot: Cleavage stimulation factor 2 Antibody [NB100-57531]
Western Blot: Cleavage stimulation factor 2 Antibody [NB100-57531] - Whole cell lysate (50 ug) from NIH3T3 cells. Antibodies: Affinity purified rabbit anti-CSTF64 antibody used for WB at 0.2 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.![Immunohistochemistry-Paraffin: Cleavage stimulation factor 2 Antibody [NB100-57531]](https://resources.rndsystems.com/images/products/Cleavage-stimulation-factor-2-Antibody-Immunohistochemistry-Paraffin-NB100-57531-img0005.jpg "Immunohistochemistry-Paraffin: Cleavage stimulation factor 2 Antibody [NB100-57531]")
Immunohistochemistry-Paraffin: Cleavage stimulation factor 2 Antibody [NB100-57531]
Immunohistochemistry-Paraffin: Cleavage stimulation factor 2 Antibody [NB100-57531] - Human ovarian carcinoma (left) and mouse teratoma (right). Antibody used at a dilution of 1:1000 (1ug/ml).![Immunoprecipitation: Cleavage stimulation factor 2 Antibody [NB100-57531]](https://resources.rndsystems.com/images/products/Cleavage-stimulation-factor-2-Antibody-Immunoprecipitation-NB100-57531-img0002.jpg "Immunoprecipitation: Cleavage stimulation factor 2 Antibody [NB100-57531]")
Immunoprecipitation: Cleavage stimulation factor 2 Antibody [NB100-57531]
Immunoprecipitation: Cleavage stimulation factor 2 Antibody [NB100-57531] - Whole cell lysate (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. NB100-57531 used for WB at 0.04 ug/ml (A) and 0.1 ug/ml (B) and used for IP at 3 ug/mg lysate. CSTF64 was also immunoprecipitated by rabbit anti-CSTF64 antibody NB100-57530, which recognizes an upstream epitope.Applications for Cleavage stimulation factor 2 Antibody
Application
Recommended Usage
Immunohistochemistry
1:100 - 1:500
Immunohistochemistry-Paraffin
1:10-1:500
Immunoprecipitation
2-5 mcg/mg
Application Notes
Immunohistochemistry 1:100 - 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: Cleavage stimulation factor 2
Alternate Names
betaCstF-64 variant 2, CF-1 64 kDa subunit, Cleavage stimulation factor 64 kDa subunit, cleavage stimulation factor subunit 2, cleavage stimulation factor, 3' pre-RNA, subunit 2, 64kD, cleavage stimulation factor, 3' pre-RNA, subunit 2, 64kDa, CSTF 64 kDa subunit, CstF-64
Entrez Gene IDs
1478 (Human)
Gene Symbol
CSTF2
UniProt
Additional Cleavage stimulation factor 2 Products
Product Documents for Cleavage stimulation factor 2 Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Cleavage stimulation factor 2 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Immunoprecipitation Protocol
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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