CLNS1A Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-33958

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Predicted:

Mouse (96%), Rat (96%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all CLNS1A Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: YTYEEGLSHLTAEGQATLERLEGMLSQSVSSQYNMAGVRTEDSIRDYEDGMEVDTTPTVAGQFEDADVDH

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for CLNS1A Antibody - BSA Free

Immunohistochemistry-Paraffin: CLNS1A Antibody [NBP2-33958]

Immunohistochemistry-Paraffin: CLNS1A Antibody [NBP2-33958]

Immunohistochemistry-Paraffin: CLNS1A Antibody [NBP2-33958] - Staining of human duodenum shows strong cytoplasmic and nuclear positivity in glandular cells.
CLNS1A Antibody - BSA Free Immunocytochemistry/ Immunofluorescence: CLNS1A Antibody [NBP2-33958]

Immunocytochemistry/ Immunofluorescence: CLNS1A Antibody [NBP2-33958]

Staining of human cell line U-2 OS shows localization to nucleoplasm & cytosol.

Applications for CLNS1A Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:20 - 1:50

Immunohistochemistry-Paraffin

1:20 - 1:50
Application Notes
IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.

Reviewed Applications

Read 1 review rated 5 using NBP2-33958 in the following applications:

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CLNS1A

The CLNS1A gene encodes a protein that functions in multiple regulatory pathways. The encoded protein complexes with numerouscytosolic proteins and performs diverse functions including regulation of small nuclear ribonucleoproteinbiosynthesis, platelet activati

Alternate Names

chloride channel regulatory protein, Chloride channel, nucleotide sensitive 1A, chloride channel, nucleotide-sensitive, 1A, Chloride conductance regulatory protein ICln, Chloride ion current inducer protein, CLCI, CLNS1B, I(Cln), ICLN, methylosome subunit pICln, Reticulocyte pICln, reticulocyte protein ICln

Gene Symbol

CLNS1A

UniProt

P54105 (Human)

Additional CLNS1A Products

Product Documents for CLNS1A Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for CLNS1A Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for CLNS1A Antibody - BSA Free

Customer Reviews for CLNS1A Antibody - BSA Free (1)

5 out of 5
1 Customer Rating
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Customer Images

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  • CLNS1A Antibody
    Name: Kathleen Mulvaney
    Application: Western Blot
    Sample Tested: Human pancreatic cancer cell lines MiaPaCa-2, AsPC-1, and BxPC-3
    Species: Human
    Verified Customer | Posted 03/20/2019
    Lane 1-AAVS1 guide RNA Lane 2-CLNS1A/pICLn targeting guide RNA
    MiaPaca2 cells stably expressing Cas9 were infected with AAVS1 control or CLNS1A/pICLn targeting guide RNA lentivirus for 48h, selected for guide RNA infection with 1ug/ml puromycin for an additional 48h. Cells were lysed in RIPA, cleared by centrifugation, soluble protein was quantified by BCA, and 20ug of whole cell extract was added in each lane. CLNS1A/pICLn was detected with this antibody at 1:2,000 dilution overnight at 4C followed by secondary antibody 1:5,000 (LiCor) for 30 minutes at room temp and developed on a LiCor imager.
    CLNS1A Antibody - BSA Free NBP2-33958

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Protocols

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FAQs

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