COP Antibody

Novus Biologicals | Catalog # NB100-856

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Peptide ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all COP Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-ETLGLSAGPIPGN corresponding to C-Terminus according to NP_443121.1.

Specificity

This antibody is expected to recognize isoform 2 (NP_443121.1) only. In addition there may be cross-reaction with INCA (GeneID 440068; NP_001007233.1).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for COP Antibody

Immunocytochemistry/ Immunofluorescence: COP Antibody [NB100-856]

Immunocytochemistry/ Immunofluorescence: COP Antibody [NB100-856]

Immunocytochemistry/Immunofluorescence: COP Antibody [NB100-856] - Analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing mitochondrial staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
Flow Cytometry: COP Antibody [NB100-856]

Flow Cytometry: COP Antibody [NB100-856]

Flow Cytometry: COP Antibody [NB100-856] - Analysis of paraformaldehyde fixed A549 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Applications for COP Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:4000
Application Notes
WB: Preliminary experiments gave an approx. 28-30 kDa band in human colon, pancreas, placenta and tonsil lysates after 0.3 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 10.7 kDa band according to NP_443121.1. The 28-30 kDa band was successfully blocked by incubation with the immunizing peptide.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: COP

Caspase inhibitor. Acts as a regulator of procaspase-1/CASP1 activation implicated in the regulation of theproteolytic maturation of pro-interleukin-1 beta (IL1B) and its release during inflammation. Inhibits the release ofIL1B in response to LPS in monocytes. Also induces NF-kappa-B activation during the pro-inflammatory cytokineresponse. Also able to inhibit CASP1-mediated neuronal cell death, TNF-alpha, hypoxia-, UV-, andstaurosporine-mediated cell death but not ER stress-mediated cell death. Acts by preventing activation of caspasesCASP1 and CASP4, possibly by preventing the interaction between CASP1 and RIPK2

Alternate Names

CARD only domain-containing protein 1, CARD only protein, caspase recruitment domain family, member 16, Caspase-1 inhibitor COP, COP1caspase-1 dominant-negative inhibitor pseudo-ICE, COPcaspase recruitment domain-containing protein 16, EC 3.4.22.36, Pseudo interleukin-1 beta converting enzyme, pseudo interleukin-1beta converting enzyme, PSEUDO-ICE

Entrez Gene IDs

114769 (Human)

Gene Symbol

CARD16

UniProt

NP_443121. (Human)

Additional COP Products

Product Documents for COP Antibody

Certificate of Analysis

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Product Specific Notices for COP Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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