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Key Product Details
Species Reactivity
Human
Applications
Western Blot, Flow Cytometry, Knockdown Validated
Label
Unconjugated
Antibody Source
Monoclonal Rabbit IgG Clone # 23GB775
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Product Specifications
Immunogen
A synthesized peptide derived from human COX4 (Uniprot: P13073)
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for COX4 Antibody (23GB775)
Flow Cytometry: COX4 Antibody (23GB775)[NBP3-52011] -
Validation of COX4 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HT-1080 cells were stained with COX4 antibody (1:2,000) and analyzed using BD flow cytometer.Flow Cytometry: COX4 Antibody (23GB775)[NBP3-52011] -
Flow cytometric analysis of COX4expression in HepG2 cells using COX4 antibody (isotype control; red, COX IV.Applications for COX4 Antibody (23GB775)
Application
Recommended Usage
Flow Cytometry
Optimal dilutions of this antibody should be experimentally determined.
Knockdown Validated
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Flow Cytometry Panel Builder
Bio-Techne Knows Flow Cytometry
Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.
Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.4), 50% glycerol
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: COX4
Long Name
Cytochrome c Oxidase Subunit IV
Alternate Names
COX4I2
Gene Symbol
COX4I1
Additional COX4 Products
Product Documents for COX4 Antibody (23GB775)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for COX4 Antibody (23GB775)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
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- Protocol: Annexin V and PI Staining by Flow Cytometry
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- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Fluorokine Flow Cytometry Kits
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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