CrkL Antibody (4B5) - Azide and BSA Free

Novus Biologicals | Catalog # H00001399-M03

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence, Proximity Ligation Assay, Knockdown Validated

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # 4B5

Format

Azide and BSA Free
View all CrkL Primary Antibodies »

Product Specifications

Immunogen

CRKL (NP_005198, 204 a.a. ~ 303 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. AHAYAQPQTTTPLPAVSGSPGAAITPLPSTQNGPVFAKAIQKRVPCAYDKTALALEVGDIVKVTRMNINGQWEGEVNGRKGLFPFTHVKIFDPQNPDENE

Specificity

CRKL (4B5)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for CrkL Antibody (4B5) - Azide and BSA Free

Western Blot: CrkL Antibody (4B5) [H00001399-M03]

Western Blot: CrkL Antibody (4B5) [H00001399-M03]

Western Blot: CrkL Antibody (4B5) [H00001399-M03] - Analysis of CRKL expression in transfected 293T cell line by CRKL monoclonal antibody (M03), clone 4B5. Lane 1: CRKL transfected lysatE (33.8 KDa). Lane 2: Non-transfected lysate.
Immunocytochemistry/ Immunofluorescence: CrkL Antibody (4B5) [H00001399-M03]

Immunocytochemistry/ Immunofluorescence: CrkL Antibody (4B5) [H00001399-M03]

Immunocytochemistry/Immunofluorescence: CrkL Antibody (4B5) [H00001399-M03] - Analysis of protein-protein interactions between PTK2 and CRKL. Huh7 cells were stained with anti-PTK2 rabbit purified polyclonal 1:600 and anti-CRKL mouse monoclonal antibody 1:100. Each red dot represents the detection of protein-protein interaction com
Western Blot: CrkL Antibody (4B5) [H00001399-M03]

Western Blot: CrkL Antibody (4B5) [H00001399-M03]

Western Blot: CrkL Antibody (4B5) [H00001399-M03] - Western blot analysis of CRKL over-expressed 293 cell line, cotransfected with CRKL Validated Chimera RNAi or non-transfected control. Blot probed with H00001399-M03. GAPDH (36.1 kDa) used as loading control.
Immunocytochemistry/ Immunofluorescence: CrkL Antibody (4B5) [H00001399-M03]

Immunocytochemistry/ Immunofluorescence: CrkL Antibody (4B5) [H00001399-M03]

Immunocytochemistry/Immunofluorescence: CrkL Antibody (4B5) [H00001399-M03] - Analysis of monoclonal antibody to CRKL on HeLa cell. Antibody concentration 10 ug/ml
Immunocytochemistry/ Immunofluorescence: CrkL Antibody (4B5) [H00001399-M03]

Immunocytochemistry/ Immunofluorescence: CrkL Antibody (4B5) [H00001399-M03]

Immunocytochemistry/Immunofluorescence: CrkL Antibody (4B5) [H00001399-M03] - Analysis of protein-protein interactions between GAB1 and CRKL. HeLa cells were stained with anti-GAB1 rabbit purified polyclonal 1:1200 and anti-CRKL mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction com
ELISA: CrkL Antibody (4B5) [H00001399-M03]

ELISA: CrkL Antibody (4B5) [H00001399-M03]

ELISA: CrkL Antibody (4B5) [H00001399-M03] - Detection limit for recombinant GST tagged CRKL is approximately 0.3ng/ml as a capture antibody.
Proximity Ligation Assay: CrkL Antibody (4B5) [H00001399-M03]

Proximity Ligation Assay: CrkL Antibody (4B5) [H00001399-M03]

Proximity Ligation Assay: CrkL Antibody (4B5) [H00001399-M03] - Analysis of protein-protein interactions between HCK and CRKL. Mahlavu cells were stained with anti-HCK rabbit purified polyclonal 1:1200 and anti-CRKL mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction co

Applications for CrkL Antibody (4B5) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB. GST tag alone is used as a negative control. It has been used for RNAi Validation.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: CrkL

v-CRK avian sarcoma virus CT10-homolog-like contains one SH2 domain and two SH3 domains. CRKL has been shown to activate the RAS and JUN kinase signaling pathways and transform fibroblasts in a RAS-dependent fashion. It is a substrate of the BCR-ABL tyrosine kinase and plays a role in fibroblast transformation by BCR-ABL. In addition, CRKL has oncogenic potential.

Long Name

V-Crk Sarcoma Virus CT10 Oncogene Homolog-like Protein

Alternate Names

crk-like protein, pCRKL, v-crk avian sarcoma virus CT10 oncogene homolog-like, v-crk sarcoma virus CT10 oncogene homolog (avian)-like

Entrez Gene IDs

1399 (Human)

Gene Symbol

CRKL

OMIM

602007 (Human)

UniProt

NP_005198 (Human)

Additional CrkL Products

Product Documents for CrkL Antibody (4B5) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for CrkL Antibody (4B5) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Apoptosis Adaptor Proteins
Interferons

Citations for CrkL Antibody (4B5) - Azide and BSA Free

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Protocols

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FAQs

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