During the G1 phase of the cell cycle, a cell must choose between rest and cell division. A small subfamily of cyclin family molecules, termed cyclin D proteins (D1, D2, D3), promotes commitment to mitosis. They accomplish this by interacting with cyclin-dependent kinases (cdk4 and cdk6) to generate Ser/Thr kinase complexes that remove blocks on DNA synthesis.
Cyclin D2 Antibody (CCND2/2620)
Novus Biologicals | Catalog # NBP3-07483
![Protein Array: Cyclin D2 Antibody (CCND2/2620) [NBP3-07483]](https://resources.rndsystems.com/images/products/Cyclin-D2-Antibody-CCND2-2620-Protein-Array-NBP3-07483-img0001.jpg "Protein Array: Cyclin D2 Antibody (CCND2/2620) [NBP3-07483]")
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Key Product Details
Species Reactivity
Human
Applications
ELISA, Protein Array
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG Kappa Clone # CCND2/2620
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Product Specifications
Immunogen
Recombinant full-length human Cyclin D2 (CCND2) protein (Uniprot: IDP30279)
Localization
Nuclear and Cytoplasmic
Clonality
Monoclonal
Host
Mouse
Isotype
IgG Kappa
Theoretical MW
34 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide.
Antibody with azide - store at 2 to 8C.
Antibody with azide - store at 2 to 8C.
Scientific Data Images for Cyclin D2 Antibody (CCND2/2620)
Protein Array: Cyclin D2 Antibody (CCND2/2620) [NBP3-07483]
Protein Array: Cyclin D2 Antibody (CCND2/2620) [NBP3-07483] - Analysis of Protein Array containing more than 19,000 full-length human proteins using Cyclin D2 Mouse Recombinant Monoclonal Antibody (CCND2/2620).Applications for Cyclin D2 Antibody (CCND2/2620)
Application
Recommended Usage
ELISA
Optimal dilutions of this antibody should be experimentally determined.
Protein Array
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
ELISA: For coating, order Ab without BSA.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: Cyclin D2
Alternate Names
CCND2, Vin1
Gene Symbol
CCND2
Additional Cyclin D2 Products
Product Documents for Cyclin D2 Antibody (CCND2/2620)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Cyclin D2 Antibody (CCND2/2620)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- Troubleshooting Guide: ELISA
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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Associated Pathways
