DACH1 Antibody

Novus Biologicals | Catalog # NBP1-00136

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Canine (100%), Mouse (100%), Porcine (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Western Blot, Peptide ELISA

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all DACH1 Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence SPVENTPQNNECK corresponding to internal region according to NP_542937.1, NP_542938.1, NP_004383.2.

Epitope

internal region

Specificity

This antibody is expected to recognize all three reported isoforms (NP_542937.1; NP_542938.1; NP_004383.2).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for DACH1 Antibody

Western Blot: DACH1 Antibody [NBP1-00136]

Western Blot: DACH1 Antibody [NBP1-00136]

Western Blot: DACH1 Antibody [NBP1-00136] - Analysis of DACH1 in Human Kidney lysate (35ug protein in RIPA buffer) using this antibody at 0.1 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence.

Applications for DACH1 Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:16000

Western Blot

0.1 - 0.3 ug/mL
Application Notes
WB: Approx. 75 and 52 kDa band bands observed in human kidney lysates (calculated MW of 73.0 kDa band according to NP_542937.1 and of 51.9 kDa band according to NP_004383.2).

Reviewed Applications

Read 1 review rated 1 using NBP1-00136 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: DACH1

This gene is similar to the Drosophila melanogaster dachshund gene, which encodes a nuclear factor essential for determining cell fates in the eye, leg, and nervous system of the fly. It is a member of the Ski gene family and is thought to be involved in eye development. Four alternatively spliced transcripts encoding different isoforms have been described for this gene. [provided by RefSeq]

Alternate Names

Dach1, DACHdachshund homolog 1, dachshund homolog (Drosophila), dachshund homolog 1 (Drosophila), FLJ10138

Entrez Gene IDs

1602 (Human); 13134 (Mouse)

Gene Symbol

DACH1

UniProt

NP_542937.1 (Human)

Additional DACH1 Products

Product Documents for DACH1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for DACH1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for DACH1 Antibody (1)

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Customer Images

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  • DACH1 Antibody
    Name: Lin Wang
    Application: Immunohistochemistry-Frozen
    Sample Tested: Mouse brain
    Species: Mouse
    Verified Customer | Posted 09/28/2021
    The Dach1 antibody did not work successfully in the E14 mouse brain and did not get any signal
    DACH1 Antibody NBP1-00136
    Bio-Techne Response
    This review was submitted through the legacy Novus Innovators Program, reflecting a new species or application tested on a primary antibody.

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