DDX5 Antibody (23GB3550)

Novus Biologicals | Catalog # NBP3-51105

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Knockdown Validated

Label

Unconjugated

Antibody Source

Monoclonal Rabbit IgG Clone # 23GB3550
View all DDX5 Primary Antibodies »

Product Specifications

Immunogen

A synthesized peptide derived from human DDX5 (Uniprot: P17844)

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for DDX5 Antibody (23GB3550)

DDX5 Antibody (23GB3550)

Immunocytochemistry/ Immunofluorescence: DDX5 Antibody (23GB3550)[NBP3-51105] -

Immunocytochemical staining of HepG2 cells with DDX5 antibody (1:1,000). Nuclei were stained blue with DAPI; DDX5 was stained magenta with Alexa Fluor(R) 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 um.
DDX5 Antibody (23GB3550)

Flow Cytometry: DDX5 Antibody (23GB3550)[NBP3-51105] -

Validation of DDX5 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with DDX5 antibody (1:2,000) and analyzed using BD flow cytometer.
DDX5 Antibody (23GB3550)

Flow Cytometry: DDX5 Antibody (23GB3550)[NBP3-51105] -

Flow cytometric analysis of DDX5expression in HepG2 cells using DDX5 antibody (1:2,000). Green, isotype control; red, DDX5.
DDX5 Antibody (23GB3550)

Western Blot: DDX5 Antibody (23GB3550)[NBP3-51105] -

Western blotting analysis using DDX5 antibody (1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using FeQ(TM) ECL Substrate Kit.
DDX5 Antibody (23GB3550)

Western Blot: DDX5 Antibody (23GB3550)[NBP3-51105] -

Western blotting analysis using DDX5 antibody (1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using FeQ(TM) ECL Substrate Kit.

Applications for DDX5 Antibody (23GB3550)

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Knockdown Validated

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry Panel Builder

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.4), 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: DDX5

DDX5, also known as Probable ATP-dependent RNA helicase DDX5, is a 69.1 kDa, 613 amino acid protein that regulates pre-mRNA splicing by increasing the inclusion of tau exon 10. Current research with the protein is being conducted for diseases and disorders such as squamous cell carcinoma, breast cancer, fibrosis, prostate cancer, hepatitis, malaria, and myeloma. The protein interacts in translational control and spliceosome pathways with HIST1H4A- HIST1H4E proteins.

Long Name

DEAD Box Protein 5

Alternate Names

DEAD/H, G17P1, HELR, HLR1, HUMP68

Gene Symbol

DDX5

Additional DDX5 Products

Product Documents for DDX5 Antibody (23GB3550)

Certificate of Analysis

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Product Specific Notices for DDX5 Antibody (23GB3550)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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