Dihydrofolate Reductase/DHFR Antibody (23GB3575)

Novus Biologicals | Catalog # NBP3-51102

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Knockdown Validated

Label

Unconjugated

Antibody Source

Monoclonal Rabbit IgG Clone # 23GB3575
View all Dihydrofolate Reductase/DHFR Primary Antibodies »

Product Specifications

Immunogen

A synthesized peptide derived from human Dihydrofolate Reductase/DHFR (Uniprot: P00374)

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Dihydrofolate Reductase/DHFR Antibody (23GB3575)

Dihydrofolate Reductase/DHFR Antibody (23GB3575)

Immunocytochemistry/ Immunofluorescence: Dihydrofolate Reductase/DHFR Antibody (23GB3575)[NBP3-51102] -

Immunocytochemical staining of Hela cells with Dihydrofolate Reductase/DHFR antibody (1:1,000). Nuclei were stained blue with DAPI; Dihydrofolate Reductase/DHFR was stained magenta with Alexa Fluor(R) 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 um.
Dihydrofolate Reductase/DHFR Antibody (23GB3575)

Flow Cytometry: Dihydrofolate Reductase/DHFR Antibody (23GB3575)[NBP3-51102] -

Validation of Dihydrofolate Reductase/DHFR knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with Dihydrofolate Reductase/DHFR antibody (1:2,000) and analyzed using BD flow cytometer.
Dihydrofolate Reductase/DHFR Antibody (23GB3575)

Flow Cytometry: Dihydrofolate Reductase/DHFR Antibody (23GB3575)[NBP3-51102] -

Flow cytometric analysis of Dihydrofolate Reductase/DHFRexpression in HeLa cells using Dihydrofolate Reductase/DHFR antibody (1:2,000). Green, isotype control; red, Dihydrofolate reductase.
Dihydrofolate Reductase/DHFR Antibody (23GB3575)

Western Blot: Dihydrofolate Reductase/DHFR Antibody (23GB3575)[NBP3-51102] -

Western blotting analysis using Dihydrofolate Reductase/DHFR antibody (1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using FeQ(TM) ECL Substrate Kit.
Dihydrofolate Reductase/DHFR Antibody (23GB3575)

Western Blot: Dihydrofolate Reductase/DHFR Antibody (23GB3575)[NBP3-51102] -

Western blotting analysis using Dihydrofolate Reductase/DHFR antibody (1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using FeQ(TM) ECL Substrate Kit.

Applications for Dihydrofolate Reductase/DHFR Antibody (23GB3575)

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Knockdown Validated

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.4), 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Dihydrofolate Reductase/DHFR

Dihydrofolate reductase converts dihydrofolate into tetrahydrofolate, a methyl group shuttle required for the de novo synthesis of purines, thymidylic acid, and certain amino acids. While the functional dihydrofolate reductase gene has been mapped to chromosome 5, multiple intronless processed pseudogenes or dihydrofolate reductase-like genes have been identified on separate chromosomes. Dihydrofolate reductase deficiency has been linked to megaloblastic anemia.

Alternate Names

DHFR, DHFRP1, DYR

Gene Symbol

DHFR

Additional Dihydrofolate Reductase/DHFR Products

Product Documents for Dihydrofolate Reductase/DHFR Antibody (23GB3575)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Dihydrofolate Reductase/DHFR Antibody (23GB3575)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Redox Enzymes

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Protocols

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